Transition from identity to bioactivity-guided proteomics for biomarker discovery with focus on the PF2D platform

Antoine Ménoret, Stephen J. Crocker, Annabelle Rodriguez-Oquendo, Vijay A. Rathinam, Robert B. Clark, Anthony T. Vella

Research output: Contribution to journalReview article

Abstract

Proteomic strategies provide a valuable tool kit to identify proteins involved in diseases. With recent progress in MS technology, high throughput proteomics has accelerated protein identification for potential biomarkers. Numerous biomarker candidates have been identified in several diseases, and many are common among pathologies. An overall strategy that could complement and strengthen the search for biomarkers is combining protein identity with biological outcomes. This review describes an emerging framework of bridging bioactivity to protein identity, exploring the possibility that some biomarkers will have a mechanistic role in the disease process. A review of pulmonary, cardiovascular, and CNS biomarkers will be discussed to demonstrate the utility of combining bioactivity with identification as a means to not only find meaningful biomarkers, but also to uncover functional mediators of disease.

Original languageEnglish (US)
Pages (from-to)8-24
Number of pages17
JournalProteomics - Clinical Applications
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Biomarkers
Bioactivity
Proteomics
Proteins
Pathology
Throughput
Technology
Lung

Keywords

  • Acute respiratory distress syndrome
  • Asthma
  • Atherosclerosis
  • Bioassay
  • Inflammation
  • Multiple sclerosis
  • Proteoform
  • PTMs
  • Validation assay

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Transition from identity to bioactivity-guided proteomics for biomarker discovery with focus on the PF2D platform. / Ménoret, Antoine; Crocker, Stephen J.; Rodriguez-Oquendo, Annabelle; Rathinam, Vijay A.; Clark, Robert B.; Vella, Anthony T.

In: Proteomics - Clinical Applications, Vol. 10, No. 1, 01.01.2016, p. 8-24.

Research output: Contribution to journalReview article

Ménoret, Antoine ; Crocker, Stephen J. ; Rodriguez-Oquendo, Annabelle ; Rathinam, Vijay A. ; Clark, Robert B. ; Vella, Anthony T. / Transition from identity to bioactivity-guided proteomics for biomarker discovery with focus on the PF2D platform. In: Proteomics - Clinical Applications. 2016 ; Vol. 10, No. 1. pp. 8-24.
@article{baba9ed7f1924811bec3abcc1a8feb67,
title = "Transition from identity to bioactivity-guided proteomics for biomarker discovery with focus on the PF2D platform",
abstract = "Proteomic strategies provide a valuable tool kit to identify proteins involved in diseases. With recent progress in MS technology, high throughput proteomics has accelerated protein identification for potential biomarkers. Numerous biomarker candidates have been identified in several diseases, and many are common among pathologies. An overall strategy that could complement and strengthen the search for biomarkers is combining protein identity with biological outcomes. This review describes an emerging framework of bridging bioactivity to protein identity, exploring the possibility that some biomarkers will have a mechanistic role in the disease process. A review of pulmonary, cardiovascular, and CNS biomarkers will be discussed to demonstrate the utility of combining bioactivity with identification as a means to not only find meaningful biomarkers, but also to uncover functional mediators of disease.",
keywords = "Acute respiratory distress syndrome, Asthma, Atherosclerosis, Bioassay, Inflammation, Multiple sclerosis, Proteoform, PTMs, Validation assay",
author = "Antoine M{\'e}noret and Crocker, {Stephen J.} and Annabelle Rodriguez-Oquendo and Rathinam, {Vijay A.} and Clark, {Robert B.} and Vella, {Anthony T.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/prca.201500029",
language = "English (US)",
volume = "10",
pages = "8--24",
journal = "Proteomics - Clinical Applications",
issn = "1862-8346",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Transition from identity to bioactivity-guided proteomics for biomarker discovery with focus on the PF2D platform

AU - Ménoret, Antoine

AU - Crocker, Stephen J.

AU - Rodriguez-Oquendo, Annabelle

AU - Rathinam, Vijay A.

AU - Clark, Robert B.

AU - Vella, Anthony T.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Proteomic strategies provide a valuable tool kit to identify proteins involved in diseases. With recent progress in MS technology, high throughput proteomics has accelerated protein identification for potential biomarkers. Numerous biomarker candidates have been identified in several diseases, and many are common among pathologies. An overall strategy that could complement and strengthen the search for biomarkers is combining protein identity with biological outcomes. This review describes an emerging framework of bridging bioactivity to protein identity, exploring the possibility that some biomarkers will have a mechanistic role in the disease process. A review of pulmonary, cardiovascular, and CNS biomarkers will be discussed to demonstrate the utility of combining bioactivity with identification as a means to not only find meaningful biomarkers, but also to uncover functional mediators of disease.

AB - Proteomic strategies provide a valuable tool kit to identify proteins involved in diseases. With recent progress in MS technology, high throughput proteomics has accelerated protein identification for potential biomarkers. Numerous biomarker candidates have been identified in several diseases, and many are common among pathologies. An overall strategy that could complement and strengthen the search for biomarkers is combining protein identity with biological outcomes. This review describes an emerging framework of bridging bioactivity to protein identity, exploring the possibility that some biomarkers will have a mechanistic role in the disease process. A review of pulmonary, cardiovascular, and CNS biomarkers will be discussed to demonstrate the utility of combining bioactivity with identification as a means to not only find meaningful biomarkers, but also to uncover functional mediators of disease.

KW - Acute respiratory distress syndrome

KW - Asthma

KW - Atherosclerosis

KW - Bioassay

KW - Inflammation

KW - Multiple sclerosis

KW - Proteoform

KW - PTMs

KW - Validation assay

UR - http://www.scopus.com/inward/record.url?scp=84953360363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953360363&partnerID=8YFLogxK

U2 - 10.1002/prca.201500029

DO - 10.1002/prca.201500029

M3 - Review article

C2 - 26201056

AN - SCOPUS:84953360363

VL - 10

SP - 8

EP - 24

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

SN - 1862-8346

IS - 1

ER -