Transient assembly of F-actin on the outer mitochondrial membrane contributes to mitochondrial fission

Sunan Li, Shan Xu, Brian A. Roelofs, Liron Boyman, W. Jonathan Lederer, Hiromi Sesaki, Mariusz Karbowski

Research output: Contribution to journalArticlepeer-review

Abstract

In addition to established membrane remodeling roles in various cellular locations, actin has recently emerged as a participant in mitochondrial fission. However, the underlying mechanisms of its participation remain largely unknown. We report that transient de novo F-actin assembly on the mitochondria occurs upon induction of mitochondrial fission and F-actin accumulates on the mitochondria without forming detectable submitochondrial foci. Impairing mitochondrial division through Drp1 knockout or inhibition prolonged the time of mitochondrial accumulation of F-actin and also led to abnormal mitochondrial accumulation of the actin regulatory factors cortactin, cofilin, and Arp2/3 complexes, suggesting that disassembly of mitochondrial F-actin depends on Drp1 activity. Furthermore, down-regulation of actin regulatory proteins led to elongation of mitochondria, associated with mitochondrial accumulation of Drp1. In addition, depletion of cortactin inhibited Mfn2 down-regulation- or FCCPinduced mitochondrial fragmentation. These data indicate that the dynamic assembly and disassembly of F-actin on the mitochondria participates in Drp1-mediated mitochondrial fission.

Original languageEnglish (US)
Pages (from-to)109-123
Number of pages15
JournalJournal of Cell Biology
Volume208
Issue number1
DOIs
StatePublished - 2015

ASJC Scopus subject areas

  • Cell Biology

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