Transgenic Plasmodium berghei sporozoites expressing β-galactosidase for quantification of sporozoite transmission

Sabine Engelmann, Photini Sinnis, Kai Matuschewski

Research output: Contribution to journalArticle

Abstract

Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here we describe the generation of Plasmodium berghei parasites that express the reporter gene lacZ as a stable transgene, under the control of the sporozoite-specific CSP promoter. Transgenic sporozoites expressing β-galactosidase can be simply visualized and quantified in an enzymatic assay. In addition, these sporozoites can be used to quantify sporozoites deposited in subcutaneous tissue during natural infection.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume146
Issue number1
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Keywords

  • LacZ
  • Plasmodium
  • Quantitative assay
  • Reporter gene
  • Sporozoite
  • β-Galactosidase

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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