Transgenic expression of interleukin-13 in the skin induces a pruritic dermatitis and skin remodeling

Tao Zheng, Min H. Oh, Sun Y. Oh, John Thomas Schroeder, Adam B. Glick, Zhou Zhu

Research output: Contribution to journalArticle

Abstract

IL-13 has been implicated in the pathogenesis of allergic diseases, including atopic dermatitis (AD). However, a direct role of IL-13 in AD has not been established. We aimed to develop an inducible transgenic model in which IL-13 can be expressed in the skin and to define the resulting dermal phenotype and mechanisms involved. The keratin 5 promoter was used with a tetracycline-inducible system to target IL-13 to the skin. The clinical manifestations, dermal histology, cytokine gene regulation, and systemic immune responses in the transgenic mice were assessed. IL-13 was produced exclusively in the skin and caused a chronic inflammatory phenotype characterized by xerosis and pruritic eczematous lesions; dermal infiltration of CD4+ T cells, mast cells, eosinophils, macrophages, and Langerhans cells; upregulation of chemokine and cytokine genes, including thymic stromal lymphopoietin; and skin remodeling with fibrosis and increased vasculature. The dermal phenotype was accompanied by elevated serum total IgE and IgG1 and increased production of IL-4 and IL-13 by CD4+ cells from lymphoid tissues and peripheral blood mononuclear cells. IL-13 is a potent stimulator of dermal inflammation and remodeling and this transgenic model of AD is a good tool for investigating the underlying mechanisms in the pathogenesis of AD.

Original languageEnglish (US)
Pages (from-to)742-751
Number of pages10
JournalJournal of Investigative Dermatology
Volume129
Issue number3
DOIs
StatePublished - Mar 2009

Fingerprint

Dermatitis
Interleukin-13
Skin
Atopic Dermatitis
Phenotype
Keratin-5
Cytokines
Histology
T-cells
Macrophages
Tetracycline
Chemokines
Infiltration
Gene expression
Interleukin-4
Immunoglobulin E
Langerhans Cells
Lymphoid Tissue
Blood
Immunoglobulin G

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Transgenic expression of interleukin-13 in the skin induces a pruritic dermatitis and skin remodeling. / Zheng, Tao; Oh, Min H.; Oh, Sun Y.; Schroeder, John Thomas; Glick, Adam B.; Zhu, Zhou.

In: Journal of Investigative Dermatology, Vol. 129, No. 3, 03.2009, p. 742-751.

Research output: Contribution to journalArticle

Zheng, Tao ; Oh, Min H. ; Oh, Sun Y. ; Schroeder, John Thomas ; Glick, Adam B. ; Zhu, Zhou. / Transgenic expression of interleukin-13 in the skin induces a pruritic dermatitis and skin remodeling. In: Journal of Investigative Dermatology. 2009 ; Vol. 129, No. 3. pp. 742-751.
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