Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates

T. M. Brushart; M. Marsel Mesulam

doi:10.1016/0304-3940(80)90051-8

Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates

T. M. Brushart, M. Marsel Mesulam

Research output: Contribution to journal › Article › peer-review

120 Scopus citations

Abstract

Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Neuroscience Letters
Volume	17
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(80)90051-8
State	Published - Apr 1980
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(80)90051-8

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title = "Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates",

abstract = "Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.",

author = "Brushart, {T. M.} and {Marsel Mesulam}, M.",

note = "Funding Information: The authors wish to thank Dr. N. Gonatas for instruction in the method of HRP-WGA conjugation and Ms. ~gina Regan for typing the manuscript. This work was supported by the Jordan Fellowship of the National Paraplegia Foundation and the Milton Fellowship of Harvard University to T.M.Bo, NSF Funding Information: grant BNS 7823610 and NIH grant NS 14625.",

year = "1980",

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doi = "10.1016/0304-3940(80)90051-8",

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T1 - Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates

AU - Brushart, T. M.

AU - Marsel Mesulam, M.

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PY - 1980/4

Y1 - 1980/4

N2 - Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.

AB - Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.

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Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates

Abstract

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