Transforming potential of deletion mutants of the SV40 T antigen coding gene in syrian hamster cells

Ricky R. Hirschhorn, W. Edward Mercer, Hui Tu Liu, Renato Baserga

Research output: Contribution to journalArticle

Abstract

Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selection and cloning, the TK-positive transformants that also expressed T antigen were tested for the extent of their transformation with respect to a number of characteristics, which included saturation density, ability to grow in soft agar, resistance to butyrate and to dibutyryl-cAMP, and plating efficiency. The combined results of these various tests indicate that cells containing partially deleted SV40 T antigen-coding genes are less transformed than cells containing an intact SV40 T antigen-coding gene. However, the amounts of T antigen are lower in cells transformed by deletion mutants than in cells transformed by wild-type T antigen-coding gene. Our data indicate that both the quantity and the quality of T antigen may be important in determining the degree of transformation in Syrian hamster cells.

Original languageEnglish (US)
Pages (from-to)220-229
Number of pages10
JournalVirology
Volume134
Issue number1
DOIs
StatePublished - Apr 15 1984
Externally publishedYes

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Polyomavirus Transforming Antigens
Mesocricetus
Viral Tumor Antigens
Thymidine Kinase
Genes
Butyrates
Human Herpesvirus 1
Agar
Organism Cloning
Plasmids

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Transforming potential of deletion mutants of the SV40 T antigen coding gene in syrian hamster cells. / Hirschhorn, Ricky R.; Edward Mercer, W.; Liu, Hui Tu; Baserga, Renato.

In: Virology, Vol. 134, No. 1, 15.04.1984, p. 220-229.

Research output: Contribution to journalArticle

Hirschhorn, Ricky R. ; Edward Mercer, W. ; Liu, Hui Tu ; Baserga, Renato. / Transforming potential of deletion mutants of the SV40 T antigen coding gene in syrian hamster cells. In: Virology. 1984 ; Vol. 134, No. 1. pp. 220-229.
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