Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain

Jasna Markovac; Gary W. Goldstein

doi:10.1016/0006-291X(88)90432-9

Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain

Jasna Markovac, Gary W. Goldstein

Kennedy Krieger Institute

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the activation of protein kinase C in microvessels isolated from rat brain. We found that unstimulated kinase activity in microvessels from immature animals is soluble while that from adults is particulate. The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the redistribution of protein kinase C activity to the membrane fraction in microvessels from immature rats. Exposure to transforming growth factor beta resulted in similar redistribution of kinase activity. To our knowledge, this is the first report of an effect of transforming growth factor beta on protein kinase C. The kinase activity in microvessels from adult animals was unaffected by exposure to these agonists. We suggest that protein kinase C activation promotes differentiation of the brain microvasculature. Transforming growth factor beta may mediate this process.

Original language	English (US)
Pages (from-to)	575-582
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(88)90432-9
State	Published - Jan 29 1988

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(88)90432-9

Fingerprint Dive into the research topics of 'Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain'. Together they form a unique fingerprint.

Cite this

@article{05f6225e95414d70bc0d2b13f0922d3f,

title = "Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain",

abstract = "We investigated the activation of protein kinase C in microvessels isolated from rat brain. We found that unstimulated kinase activity in microvessels from immature animals is soluble while that from adults is particulate. The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the redistribution of protein kinase C activity to the membrane fraction in microvessels from immature rats. Exposure to transforming growth factor beta resulted in similar redistribution of kinase activity. To our knowledge, this is the first report of an effect of transforming growth factor beta on protein kinase C. The kinase activity in microvessels from adult animals was unaffected by exposure to these agonists. We suggest that protein kinase C activation promotes differentiation of the brain microvasculature. Transforming growth factor beta may mediate this process.",

author = "Jasna Markovac and Goldstein, {Gary W.}",

year = "1988",

month = jan,

day = "29",

doi = "10.1016/0006-291X(88)90432-9",

language = "English (US)",

volume = "150",

pages = "575--582",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Transforming growth factor beta activates protein kinase C in microvessels isolated from immature rat brain

AU - Markovac, Jasna

AU - Goldstein, Gary W.

PY - 1988/1/29

Y1 - 1988/1/29

N2 - We investigated the activation of protein kinase C in microvessels isolated from rat brain. We found that unstimulated kinase activity in microvessels from immature animals is soluble while that from adults is particulate. The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the redistribution of protein kinase C activity to the membrane fraction in microvessels from immature rats. Exposure to transforming growth factor beta resulted in similar redistribution of kinase activity. To our knowledge, this is the first report of an effect of transforming growth factor beta on protein kinase C. The kinase activity in microvessels from adult animals was unaffected by exposure to these agonists. We suggest that protein kinase C activation promotes differentiation of the brain microvasculature. Transforming growth factor beta may mediate this process.

AB - We investigated the activation of protein kinase C in microvessels isolated from rat brain. We found that unstimulated kinase activity in microvessels from immature animals is soluble while that from adults is particulate. The tumor promoter, phorbol 12-myristate 13-acetate, and the diacylglycerol analog, 1-oleoyl-2-acetyl-sn-glycerol, caused the redistribution of protein kinase C activity to the membrane fraction in microvessels from immature rats. Exposure to transforming growth factor beta resulted in similar redistribution of kinase activity. To our knowledge, this is the first report of an effect of transforming growth factor beta on protein kinase C. The kinase activity in microvessels from adult animals was unaffected by exposure to these agonists. We suggest that protein kinase C activation promotes differentiation of the brain microvasculature. Transforming growth factor beta may mediate this process.

UR - http://www.scopus.com/inward/record.url?scp=0023851621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023851621&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(88)90432-9

DO - 10.1016/0006-291X(88)90432-9

M3 - Article

C2 - 3342038

AN - SCOPUS:0023851621

VL - 150

SP - 575

EP - 582

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -