Transforming growth factor-β increases Escherichia coli K1 adherence, invasion, and transcytosis in human brain microvascular endothelial cells

Guang Zhang, Naveed Khan, Kee Kim, Monique Stins, Kwang Kim

Research output: Contribution to journalArticle

Abstract

Escherichia coli K1 traversal of the human brain microvascular endothelial cells (HBMEC) that constitute the blood-brain barrier (BBB) is a complex process involving E. coli adherence to and invasion of HBMEC. In this study, we demonstrated that human transforming growth factor-beta-1 (TGF-β1) increases E. coli K1 adherence, invasion, and transcytosis in HBMEC. In addition, TGF-β1 increases RhoA activation and enhances actin condensation in HBMEC. We have previously shown that E. coli K1 invasion of HBMEC requires phosphatidylinositol-3 kinase (PI3K) and RhoA activation. TGF-β1 increases E. coli K1 invasion in PI3K dominant-negative HBMEC, but not in RhoA dominant-negative HBMEC, indicating that TGF-β1-mediated increase in E. coli K1 invasion is RhoA-dependent, but not PI3K-dependent. Our findings suggest that TGF-β1 treatment of HBMEC increases E. coli K1 adherence, invasion, and transcytosis, which are probably dependent on RhoA.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalCell and Tissue Research
Volume309
Issue number2
DOIs
StatePublished - Sep 12 2002

Keywords

  • Cell culture
  • Escherichia coli
  • Human brain microvascular endothelial cells
  • RhoA
  • Transforming growth factor-beta-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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