Abstract
The transforming growth factor-βs are peptide growth factors known to play a central role in wound healing. Using a specific, in vitro assay of cell growth inhibition, we have detected transforming growth factor-β (TGF-β in 24/24 aqueous humor specimens from eyes undergoing cataract extraction with intraocular lens implantation. The amount of TGF-β ranged from 2.3 to 8.1 ng/ml (mean ± SD = 4.5 ± 1.7 ng/ml), with 61% present in the active form. Subtyping of TGF-β was performed by addition of antibodies specific for the ß1 and ß2 isoforms to the growth inhibition assay, and confirmed with a sandwich enzyme-linked immunosorbent assay. None of the TGF-β detected was of the ß1 isoform; in contrast, the ß2 isoform was present in every sample, implying that it might have originated from ocular tissues. The presence of this potent modulator of tissue repair in aqueous humor suggests a role in the healing processes following intraocular surgery, including glaucoma filtration surgery.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 963-969 |
| Number of pages | 7 |
| Journal | Current Eye Research |
| Volume | 9 |
| Issue number | 10 |
| DOIs | |
| State | Published - 1990 |
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience