Transforming growth factor-β expression in human retinal pigment epithelial cells is enhanced by Toxoplasma gondii: A possible role in the immunopathogenesis of retinochoroiditis

C. N. Nagineni, Barbara Detrick, J. J. Hooks

Research output: Contribution to journalArticle

Abstract

Retinochoroiditis caused by Toxoplasma gondii infection results in inflammation and necrosis of the retina. We have used human retinal pigment epithelial cultures (HRPE) as an in vitro model to investigate the role of TGF-β in T. gondii-induced retinochoroiditis. RT-PCR analyses showed enhanced steady state levels of TGF-β1 and TGF-β2 mRNA in T. gondii-infected HRPE. Uninfected HRPE secrete TGF-β1 in a latent form while 10-30% of the secreted TGF-β2 was in the active form. T. gondii infection induced a significant increase (P

Original languageEnglish (US)
Pages (from-to)372-378
Number of pages7
JournalClinical and Experimental Immunology
Volume128
Issue number2
DOIs
StatePublished - 2002

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Retinal Pigments
Toxoplasma
Transforming Growth Factors
Epithelial Cells
Toxoplasmosis
Retina
Necrosis
Inflammation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Immunopathogenesis
  • Retinochoroiditis
  • TGF-β
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Immunology

Cite this

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abstract = "Retinochoroiditis caused by Toxoplasma gondii infection results in inflammation and necrosis of the retina. We have used human retinal pigment epithelial cultures (HRPE) as an in vitro model to investigate the role of TGF-β in T. gondii-induced retinochoroiditis. RT-PCR analyses showed enhanced steady state levels of TGF-β1 and TGF-β2 mRNA in T. gondii-infected HRPE. Uninfected HRPE secrete TGF-β1 in a latent form while 10-30{\%} of the secreted TGF-β2 was in the active form. T. gondii infection induced a significant increase (P",
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