Transformation with katG restores isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations

Ying Zhang; Thomas Garbe; Douglas Young

doi:10.1111/j.1365-2958.1993.tb01596.x

Transformation with katG restores isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations

Ying Zhang, Thomas Garbe, Douglas Young

Research output: Contribution to journal › Article › peer-review

153 Scopus citations

Abstract

Isoniazid‐resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase‐peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50μgml⁻¹. Transformation with the corresponding katG gene from Escherichia coli resulted in low‐level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.

Original language	English (US)
Pages (from-to)	521-524
Number of pages	4
Journal	Molecular Microbiology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-2958.1993.tb01596.x
State	Published - May 1993
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1111/j.1365-2958.1993.tb01596.x

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title = "Transformation with katG restores isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations",

abstract = "Isoniazid‐resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase‐peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50μgml−1. Transformation with the corresponding katG gene from Escherichia coli resulted in low‐level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.",

author = "Ying Zhang and Thomas Garbe and Douglas Young",

year = "1993",

month = may,

doi = "10.1111/j.1365-2958.1993.tb01596.x",

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journal = "Molecular Microbiology",

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AU - Zhang, Ying

AU - Garbe, Thomas

AU - Young, Douglas

PY - 1993/5

Y1 - 1993/5

N2 - Isoniazid‐resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase‐peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50μgml−1. Transformation with the corresponding katG gene from Escherichia coli resulted in low‐level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.

AB - Isoniazid‐resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase‐peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to >50μgml−1. Transformation with the corresponding katG gene from Escherichia coli resulted in low‐level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.

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Transformation with katG restores isoniazid‐sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations

Abstract

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