Transfer of a functional arabinose operator-repressor system to Drosophila melanogaster Schneider line-2 cells

Venu Raman, Keith N. Rand, Ronald J. Hill

Research output: Contribution to journalArticlepeer-review

Abstract

Regulatory elements from the arabinose operon of Escherichia coli were transfected into Drosophila melanogaster Schneider line 2 cells to test their ability to function in animal cells. A construct containing an araC fusion gene (encoding AraC protein) under the control of an act5C (encoding actin) promoter and a construct containing a lacZ fusion gene (reporter gene) also under the control of an act5C promoter were used to build a regulatory circuit in the D. melanogaster cells. We have demonstrated that a AraC fusion protein can be synthesised in Schneider cells where it is able to repress the transcription of the reporter gene containing AraC-binding sites inserted between the transcription start point and the initiation codon. The reporter gene activity can be further modulated by the addition of arabinose to the medium.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalBBA - Gene Structure and Expression
Volume1219
Issue number2
DOIs
StatePublished - Oct 18 1994
Externally publishedYes

Keywords

  • AraC protein
  • Gene expression
  • Protein-DNA interaction
  • Regulatory circuit
  • Transfection

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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