Abstract
Regulatory elements from the arabinose operon of Escherichia coli were transfected into Drosophila melanogaster Schneider line 2 cells to test their ability to function in animal cells. A construct containing an araC fusion gene (encoding AraC protein) under the control of an act5C (encoding actin) promoter and a construct containing a lacZ fusion gene (reporter gene) also under the control of an act5C promoter were used to build a regulatory circuit in the D. melanogaster cells. We have demonstrated that a AraC fusion protein can be synthesised in Schneider cells where it is able to repress the transcription of the reporter gene containing AraC-binding sites inserted between the transcription start point and the initiation codon. The reporter gene activity can be further modulated by the addition of arabinose to the medium.
Original language | English (US) |
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Pages (from-to) | 441-448 |
Number of pages | 8 |
Journal | BBA - Gene Structure and Expression |
Volume | 1219 |
Issue number | 2 |
DOIs | |
State | Published - Oct 18 1994 |
Externally published | Yes |
Keywords
- AraC protein
- Gene expression
- Protein-DNA interaction
- Regulatory circuit
- Transfection
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics