Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis

Karen A. Munger, Angel Montero, Megumu Fukunaga, Susumu Uda, Takafumi Yura, Enyu Imai, Yasufumi Kaneda, José M. Valdivielso, Kamal F. Badr

Research output: Contribution to journalArticle

Abstract

The human 15-lipoxygenase (15-LO) gene was transfected into rat kidneys in vivo via intra-renal arterial injection. Three days later, acute (passive) or accelerated forms of antiglomerular basement membrane antibody-mediated glomerulonephritis were induced in transfected and nontransfected or sham- transfected controls. Studies of glomerular functions (filtration and protein excretion) and ex vivo glomerular leukotriene B4 biosynthesis at 3 hr, and up to 4 days, after induction of nephritis revealed preservation or normalization of these parameters in transfected kidneys that expressed human 15-LO mRNA and mature protein, but not in contralateral control kidneys or sham-transfected animals. The results provide in vivo-derived data supporting a direct antiinflammatory role for 15-LO during immune-mediated tissue injury.

Original languageEnglish (US)
Pages (from-to)13375-13380
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number23
DOIs
StatePublished - Nov 9 1999
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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