TY - JOUR
T1 - Transduction of TAT fusion proteins into osteoclasts and osteoblasts
AU - Dolgilevich, Svetlana
AU - Zaidi, Neeha
AU - Song, Jianbo
AU - Abe, Etsuko
AU - Moonga, Baljit S.
AU - Sun, Li
N1 - Funding Information:
Li Sun, MD, PhD, thanks the National Institutes of Health for Training Grant support. The work was supported, in part, by the Geriatrics Research Education and Clinical Center of the Bronx Veterans Affairs Medical Center, New York.
PY - 2002
Y1 - 2002
N2 - It has been difficult to transduce primary cultures of bone cells with proteins of interest. Here, we report the development and validation of a new technology for transduction of osteoblasts and osteoclasts with peptides and moderately sized proteins. Fusion proteins between TAT, an 11 amino acid Arg-rich sequence derived from the HIV protein, and either hemagglutinin or calcineurin Aα were synthesized and purified. Exposure of osteoblasts and osteoclasts in primary culture to either TAT-HA or TAT-calcineurin Aα resulted in a rapid (within 10 min) intracellular movement of the fusion protein evident on co-immunostaining. Almost 99% of cells were transduced and the fusion protein was retained in ∼50% of the cells for up to 5 days. TAT did not abolish the functionality of calcineurin Aα; the fusion protein stimulated osteoblast differentiation and inhibited osteoclastic resorption. We expect that our studies will provide a firm basis for the future development of TAT fusion proteins for critical molecules involved in bone cell differentiation and function.
AB - It has been difficult to transduce primary cultures of bone cells with proteins of interest. Here, we report the development and validation of a new technology for transduction of osteoblasts and osteoclasts with peptides and moderately sized proteins. Fusion proteins between TAT, an 11 amino acid Arg-rich sequence derived from the HIV protein, and either hemagglutinin or calcineurin Aα were synthesized and purified. Exposure of osteoblasts and osteoclasts in primary culture to either TAT-HA or TAT-calcineurin Aα resulted in a rapid (within 10 min) intracellular movement of the fusion protein evident on co-immunostaining. Almost 99% of cells were transduced and the fusion protein was retained in ∼50% of the cells for up to 5 days. TAT did not abolish the functionality of calcineurin Aα; the fusion protein stimulated osteoblast differentiation and inhibited osteoclastic resorption. We expect that our studies will provide a firm basis for the future development of TAT fusion proteins for critical molecules involved in bone cell differentiation and function.
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U2 - 10.1016/S0006-291X(02)02664-5
DO - 10.1016/S0006-291X(02)02664-5
M3 - Article
C2 - 12445831
AN - SCOPUS:0036434587
SN - 0006-291X
VL - 299
SP - 505
EP - 509
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -