Transduction of Surface and Basal Cells in Rhesus Macaque Lung following Repeat Dosing with AAV1CFTR

William B. Guggino, Murali K. Yanda, Cristina V. Cebotaru, Liudmila Cebotaru

Research output: Contribution to journalArticlepeer-review

Abstract

To test the effectiveness of repeat dosing, we sprayed two doses (1013 vg each) of AAV1Δ27-264-CFTR into airways of four rhesus monkeys at 0 and 30 days, followed by a single dose of 1013 vg of AAV1GFP on day 60. Monkeys were sacrificed on day 90. No adverse events occurred, indicating that AAV1 vectors are safe. An elevated anti-AAV1 neutralizing titer was established by the third dose. A positive ELISPOT to the adeno-associated virus (AAV) capsid but not to cystic fibrosis transmembrane conductance regulator (CFTR) occurred after the third dose in three monkeys. AAV1-CFTR and GFP vectors were detectable in all lung sections and in the heart, liver, and spleen. The CFTR protein was higher in treated monkeys than in an untreated monkey. GFP protein was detected in treated lungs. Lung surface and keratin 5-positive basal cells showed higher CFTR staining than in the uninfected monkey and were positive for GFP staining, indicating widespread gene transduction by AAV1CFTR and GFP. AAV1 safely and effectively transduces monkey airway and basal cells. Both the significant numbers of vector genomes and transduction from AAV1CFTR and GFP virus seen in the monkeys 3 months after the first instillation suggest that repeat dosing with AAV1-based vectors is achievable.

Original languageEnglish (US)
Pages (from-to)1010-1023
Number of pages14
JournalHuman gene therapy
Volume31
Issue number17-18
DOIs
StatePublished - Sep 2020

Keywords

  • AAV1
  • CFTR
  • basal cells
  • cystic fibrosis
  • lung
  • repeat dosing
  • rhesus
  • surface cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Transduction of Surface and Basal Cells in Rhesus Macaque Lung following Repeat Dosing with AAV1CFTR'. Together they form a unique fingerprint.

Cite this