Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest

Rubén Martínez-Barricarte, Sarah Jill De Jong, Janet Markle, Roel De Paus, Stephanie Boisson-Dupuis, Jacinta Bustamante, Esther Van de Vosse, Bernhard Fleckenstein, Jean Laurent Casanova

Research output: Contribution to journalArticlepeer-review

Abstract

Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described.

Original languageEnglish (US)
Pages (from-to)7.21C.1-7.21C.12
JournalCurrent protocols in immunology
Volume2016
DOIs
StatePublished - 2016

Keywords

  • HVS-transformed T cell
  • Herpesvirus saimiri-transformed T cells
  • Retrovirus
  • T cells
  • Transduction

ASJC Scopus subject areas

  • Immunology

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