Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest

Rubén Martínez-Barricarte, Sarah Jill De Jong, Janet Markle, Roel De Paus, Stephanie Boisson-Dupuis, Jacinta Bustamante, Esther Van de Vosse, Bernhard Fleckenstein, Jean Laurent Casanova

Research output: Contribution to journalArticle

Abstract

Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described.

Original languageEnglish (US)
Pages (from-to)7.21C.1-7.21C.12
JournalCurrent Protocols in Immunology
Volume2016
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Saimiriine Herpesvirus 2
T-Lymphocytes
Genes
Retroviridae
Alleles
Gene Expression

Keywords

  • Herpesvirus saimiri-transformed T cells
  • HVS-transformed T cell
  • Retrovirus
  • T cells
  • Transduction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Martínez-Barricarte, R., De Jong, S. J., Markle, J., De Paus, R., Boisson-Dupuis, S., Bustamante, J., ... Casanova, J. L. (2016). Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest. Current Protocols in Immunology, 2016, 7.21C.1-7.21C.12. https://doi.org/10.1002/cpim.15

Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest. / Martínez-Barricarte, Rubén; De Jong, Sarah Jill; Markle, Janet; De Paus, Roel; Boisson-Dupuis, Stephanie; Bustamante, Jacinta; Van de Vosse, Esther; Fleckenstein, Bernhard; Casanova, Jean Laurent.

In: Current Protocols in Immunology, Vol. 2016, 01.01.2016, p. 7.21C.1-7.21C.12.

Research output: Contribution to journalArticle

Martínez-Barricarte, R, De Jong, SJ, Markle, J, De Paus, R, Boisson-Dupuis, S, Bustamante, J, Van de Vosse, E, Fleckenstein, B & Casanova, JL 2016, 'Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest', Current Protocols in Immunology, vol. 2016, pp. 7.21C.1-7.21C.12. https://doi.org/10.1002/cpim.15
Martínez-Barricarte R, De Jong SJ, Markle J, De Paus R, Boisson-Dupuis S, Bustamante J et al. Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest. Current Protocols in Immunology. 2016 Jan 1;2016:7.21C.1-7.21C.12. https://doi.org/10.1002/cpim.15
Martínez-Barricarte, Rubén ; De Jong, Sarah Jill ; Markle, Janet ; De Paus, Roel ; Boisson-Dupuis, Stephanie ; Bustamante, Jacinta ; Van de Vosse, Esther ; Fleckenstein, Bernhard ; Casanova, Jean Laurent. / Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest. In: Current Protocols in Immunology. 2016 ; Vol. 2016. pp. 7.21C.1-7.21C.12.
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