TY - JOUR
T1 - Transduction of herpesvirus saimiri-transformed T cells with exogenous genes of interest
AU - Martínez-Barricarte, Rubén
AU - De Jong, Sarah Jill
AU - Markle, Janet
AU - De Paus, Roel
AU - Boisson-Dupuis, Stephanie
AU - Bustamante, Jacinta
AU - Van de Vosse, Esther
AU - Fleckenstein, Bernhard
AU - Casanova, Jean Laurent
N1 - Funding Information:
RMB was funded by an EMBO long-term fellowship, SJdJ by a research fellowship from the German research foundation (DFG), JM by a fellowship from the Canadian Institutes of Health Research (CIHR) and the Charles H. Revson Senior Fellowship in Biomedical Sciences. This work was funded by the St. Giles Foundation, the Rockefeller University, Insti-tut National de la Santé et de la Recherche Médicale (INSERM), Paris Descartes University and the French National Research Agency (ANR), under the “Investments for the future” program (grant no. ANR-10-IAHU-01).
Publisher Copyright:
© 2016 by John Wiley & Sons, Inc.
PY - 2016
Y1 - 2016
N2 - Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described.
AB - Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described.
KW - HVS-transformed T cell
KW - Herpesvirus saimiri-transformed T cells
KW - Retrovirus
KW - T cells
KW - Transduction
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U2 - 10.1002/cpim.15
DO - 10.1002/cpim.15
M3 - Article
C2 - 27801513
AN - SCOPUS:85029409873
SN - 1934-3671
VL - 2016
SP - 7.21C.1-7.21C.12
JO - Current protocols in immunology
JF - Current protocols in immunology
ER -