@article{bd894e50d17e481d86d5b1f8576799db,
title = "Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites",
abstract = "Plasmodium sporozoites are transmitted from infected mosquitoes to mammals, and must navigate the host skin and vasculature to infect the liver. This journey requires distinct proteomes. Here, we report the dynamic transcriptomes and proteomes of both oocyst sporozoites and salivary gland sporozoites in both rodent-infectious Plasmodium yoelii parasites and human-infectious Plasmodium falciparum parasites. The data robustly define mRNAs and proteins that are upregulated in oocyst sporozoites (UOS) or upregulated in infectious sporozoites (UIS) within the salivary glands, including many that are essential for sporozoite functions in the vector and host. Moreover, we find that malaria parasites use two overlapping, extensive, and independent programs of translational repression across sporozoite maturation to temporally regulate protein expression. Together with gene-specific validation experiments, these data indicate that two waves of translational repression are implemented and relieved at different times during sporozoite maturation, migration and infection, thus promoting their successful development and vector-to-host transition.",
author = "Lindner, {Scott E.} and Swearingen, {Kristian E.} and Shears, {Melanie J.} and Walker, {Michael P.} and Vrana, {Erin N.} and Hart, {Kevin J.} and Minns, {Allen M.} and Photini Sinnis and Moritz, {Robert L.} and Kappe, {Stefan H.I.}",
note = "Funding Information: We appreciate and acknowledge ongoing scientific discussions with Istvan Albert, Aswathy Sebastian, Manuel Llin{\'a}s and his research group, Kelly Rios for graphic design assistance, as well as advice given on sequencing services provided by the Penn State Genomics Core Facility (University Park, PA). The authors would like to thank the Johns Hopkins Malaria Research Institute Insectary and Parasitology core facilities, particularly, Christopher Kizito for expert rearing of mosquitoes, and Drs. Abhai Tripathi and Godfree Mlambo for production of the P. falciparum-infected mosquitoes. We are grateful to Bloomberg Philanthropies for support of these core facilities. Research reported in this publication was supported by Penn State start-up funds (SEL), the National Institutes of Health National Institute of Allergy and Infectious Disease (http:// www.niaid.nih.gov/) under award numbers 1K22AI101039 (S.E.L.), 1R01AI123341 (S.E.L.), K25AI119229 (K.E.S.), R01AI132359 (P.S.), and R01AI134956 (S.H.I.K.), by the National Institutes of Health National Institute of General Medical Sciences (www.nigms. nih.gov) under award number R01GM087221 (R.L.M.), by the National Institutes of Health National Center for Research Resources under award number S10RR027584 (R.L. M.), by the National Science Foundation (www.nsf.gov) under award number 0923536 (R.L.M. and K.E.S.), and by the Provost{\textquoteright}s Postdoctoral Diversity Fellowship from Johns Hopkins University (M.S.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, the National Science Foundation or the Bill and Melinda Gates Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the paper. Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41467-019-12936-6",
language = "English (US)",
volume = "10",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}