Transcriptome studies of the floodwater mosquito, Aedes vexans (Diptera: Culicidae) with potential as secondary vectors using Illumina HiSeq 4,000 sequencing

Jong Min Chung, Jie Eun Park, Hee Ju Hwang, Min Kyu Sang, Hye Rin Min, Hang Chul Cho, Se Won Kang, So Young Park, Snigdha Baliarsingh, Hong Seog Park, Sung Jae Cha, E. Hyun Shin, Bharat Bhusan Patnaik, Yong Seok Lee

Research output: Contribution to journalArticle

Abstract

Aedes vexans is the inland floodwater mosquito widely distributed in every continent excepting Antarctica and South America. They are opportunistic feeders preferring blood meal of larger animals including cattle, horses, deer, and humans. Further, the mosquito species is a compatible vector of several diseases, including West Nile virus and dog heartworm. In this study, we performed transcriptome characterization of Ae. vexans using Illumina HiSeq 4,000 sequencing and assembly of sequenced reads using Trinity. A total of 55,813,852 raw read and 54,630,771 clean reads (97.88% of raw reads) were obtained after Illumina paired-end sequencing and pre-processing steps. After Trinity de novo assembly, TransDecoder and TGICL clustering, a total of 37,111 unigenes were obtained. Out of the total unigenes count, 28,733, 17,893, 14,626, and 17,055 showed homologous matches against the PANM, UniGene, SwissProt, and KOG databases. A total of 9,483 unigenes were assigned to Gene Ontology (GO) terms, and 3,741 unigenes were mapped to 483 KEGG pathways. The zinc finger (C2H2-type), reverse transcriptase, integrase (catalytic core), protein kinase, and RNA recognition motif domain among others showed as the top InterProScan domains. The obtained datasets serves as a basis for future studies towards understanding ecology, metabolism, and parasitism potential of Ae. vexans.

Original languageEnglish (US)
JournalEntomological Research
DOIs
StateAccepted/In press - 2020

Keywords

  • Aedes vexans
  • Trinity assembly
  • transcriptome

ASJC Scopus subject areas

  • Insect Science

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    Chung, J. M., Park, J. E., Hwang, H. J., Sang, M. K., Min, H. R., Cho, H. C., Kang, S. W., Park, S. Y., Baliarsingh, S., Park, H. S., Cha, S. J., Shin, E. H., Patnaik, B. B., & Lee, Y. S. (Accepted/In press). Transcriptome studies of the floodwater mosquito, Aedes vexans (Diptera: Culicidae) with potential as secondary vectors using Illumina HiSeq 4,000 sequencing. Entomological Research. https://doi.org/10.1111/1748-5967.12440