TY - JOUR
T1 - Transcriptome studies of the floodwater mosquito, Aedes vexans (Diptera: Culicidae) with potential as secondary vectors using Illumina HiSeq 4,000 sequencing
AU - Chung, Jong Min
AU - Park, Jie Eun
AU - Hwang, Hee Ju
AU - Sang, Min Kyu
AU - Min, Hye Rin
AU - Cho, Hang Chul
AU - Kang, Se Won
AU - Park, So Young
AU - Baliarsingh, Snigdha
AU - Park, Hong Seog
AU - Cha, Sung Jae
AU - Shin, E. Hyun
AU - Patnaik, Bharat Bhusan
AU - Lee, Yong Seok
N1 - Funding Information:
This study was supported by Government‐wide R&D Fund project for infectious disease research, HG18C0028020019 and Soonchunhyang University Research Fund.
Publisher Copyright:
© 2020 The Entomological Society of Korea and John Wiley & Sons Australia, Ltd
PY - 2020/12
Y1 - 2020/12
N2 - Aedes vexans is the inland floodwater mosquito widely distributed in every continent excepting Antarctica and South America. They are opportunistic feeders preferring blood meal of larger animals including cattle, horses, deer, and humans. Further, the mosquito species is a compatible vector of several diseases, including West Nile virus and dog heartworm. In this study, we performed transcriptome characterization of Ae. vexans using Illumina HiSeq 4,000 sequencing and assembly of sequenced reads using Trinity. A total of 55,813,852 raw read and 54,630,771 clean reads (97.88% of raw reads) were obtained after Illumina paired-end sequencing and pre-processing steps. After Trinity de novo assembly, TransDecoder and TGICL clustering, a total of 37,111 unigenes were obtained. Out of the total unigenes count, 28,733, 17,893, 14,626, and 17,055 showed homologous matches against the PANM, UniGene, SwissProt, and KOG databases. A total of 9,483 unigenes were assigned to Gene Ontology (GO) terms, and 3,741 unigenes were mapped to 483 KEGG pathways. The zinc finger (C2H2-type), reverse transcriptase, integrase (catalytic core), protein kinase, and RNA recognition motif domain among others showed as the top InterProScan domains. The obtained datasets serves as a basis for future studies towards understanding ecology, metabolism, and parasitism potential of Ae. vexans.
AB - Aedes vexans is the inland floodwater mosquito widely distributed in every continent excepting Antarctica and South America. They are opportunistic feeders preferring blood meal of larger animals including cattle, horses, deer, and humans. Further, the mosquito species is a compatible vector of several diseases, including West Nile virus and dog heartworm. In this study, we performed transcriptome characterization of Ae. vexans using Illumina HiSeq 4,000 sequencing and assembly of sequenced reads using Trinity. A total of 55,813,852 raw read and 54,630,771 clean reads (97.88% of raw reads) were obtained after Illumina paired-end sequencing and pre-processing steps. After Trinity de novo assembly, TransDecoder and TGICL clustering, a total of 37,111 unigenes were obtained. Out of the total unigenes count, 28,733, 17,893, 14,626, and 17,055 showed homologous matches against the PANM, UniGene, SwissProt, and KOG databases. A total of 9,483 unigenes were assigned to Gene Ontology (GO) terms, and 3,741 unigenes were mapped to 483 KEGG pathways. The zinc finger (C2H2-type), reverse transcriptase, integrase (catalytic core), protein kinase, and RNA recognition motif domain among others showed as the top InterProScan domains. The obtained datasets serves as a basis for future studies towards understanding ecology, metabolism, and parasitism potential of Ae. vexans.
KW - Aedes vexans
KW - Trinity assembly
KW - transcriptome
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U2 - 10.1111/1748-5967.12440
DO - 10.1111/1748-5967.12440
M3 - Article
AN - SCOPUS:85087307119
SN - 1738-2297
VL - 50
SP - 563
EP - 574
JO - Entomological Research
JF - Entomological Research
IS - 12
ER -