TY - JOUR
T1 - Transcriptome of the adult female malaria mosquito vector Anopheles albimanus.
AU - Martínez-Barnetche, Jesús
AU - Gómez-Barreto, Rosa E.
AU - Ovilla-Muñoz, Marbella
AU - Téllez-Sosa, Juan
AU - López, David E.García
AU - Dinglasan, Rhoel R.
AU - Mohien, Ceereena Ubaida
AU - MacCallum, Robert M.
AU - Redmond, Seth N.
AU - Gibbons, John G.
AU - Rokas, Antonis
AU - Machado, Carlos A.
AU - Cazares-Raga, Febe E.
AU - González-Cerón, Lilia
AU - Hernández-Martínez, Salvador
AU - López, Mario H.Rodríguez
N1 - Funding Information:
We thank Gabriel Hernandez of ROCHE technical support for help with 454 sequencing; Travis Clark and Chelsea Baker of the Vanderbilt Genome Technology Core for help with Illumina sequencing; Bruno Arce for SQL database support, Bernardo Cortina and Kelly Scoggins for manuscript assistance; and Fidel de la Cruz Hernandez for critically reviewing the manuscript. The authors are very grateful to Imperial College London for high performance computing facilities. We also thank the anonymous reviewers for their suggestions improving the manuscript. This investigation received financial support from the UNICEF/UNDP/WORLD BANK/WHO Special Programme for Research and Training in Tropical Diseases (TDR), project ID: A50256 (for JMB) and CONACyT-SEP-2007SEP-62389 (for MHR); from the Bloomberg Family Foundation and the Johns Hopkins Malaria Research Institute (JHMRI) (for RRD) and grant HHSN268201000032C (N01-HV-00240) from NHLBI, NIH (CU); the Advanced Computing Center for Research and Education at Vanderbilt University. JGG is funded by the Graduate Program in Biological Sciences at Vanderbilt University and the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH, NIAID: F31AI091343-01). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIAID or the NIH. Research in AR’s lab is supported by the Searle Scholars Program and the National Science Foundation (DEB-0844968). VectorBase is supported by National Institutes of Health/National Institute for Allergy and Infectious Diseases contracts HHSN266200400039C and HSN272200900039C). Experimental mosquito infections received support from the CONACYT SALUD-2004-C01-119 grant (for LGC).
PY - 2012
Y1 - 2012
N2 - Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 My. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex. We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bidirectional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects. We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).
AB - Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 My. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex. We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bidirectional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects. We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).
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U2 - 10.1186/1471-2164-13-207
DO - 10.1186/1471-2164-13-207
M3 - Article
C2 - 22646700
AN - SCOPUS:84861533252
VL - 13
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
ER -