Transcriptome of the adult female malaria mosquito vector Anopheles albimanus

Jesus Martinez-Barnetche, Rosa E. Gómez-Barreto, Marbella Ovilla-Muñoz, Juan Téllez-Sosa, David E. García-López, Rhoel R. Dinglasan, Ceereena Ubaida Mohien, Robert M. MacCallum, Seth N. Redmond, John G. Gibbons, Antonis Rokas, Carlos M. Machado, Febe Cazares-Raga, Lilia González-Cerón, Salvador Hernández-Martínez, Mario H. Rodríguez-Lopez

Research output: Contribution to journalArticle

Abstract

Background: Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 million years. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex. Results: We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bi-directional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects. Conclusions: We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).

Original languageEnglish (US)
Pages (from-to)207
Number of pages1
JournalBMC Genomics
DOIs
StateAccepted/In press - May 30 2012
Externally publishedYes

Fingerprint

Anopheles
Transcriptome
Malaria
Culicidae
Anopheles gambiae
Plasmodium
Genome
Entomology
Insect Vectors
Biological Phenomena
Proteins
Fat Body
Biological Factors
Proteome
Genomics
Salivary Glands
Gene Library
Proteomics
Mosquito Vectors
Biomedical Research

ASJC Scopus subject areas

  • Genetics
  • Biotechnology

Cite this

Martinez-Barnetche, J., Gómez-Barreto, R. E., Ovilla-Muñoz, M., Téllez-Sosa, J., García-López, D. E., Dinglasan, R. R., ... Rodríguez-Lopez, M. H. (Accepted/In press). Transcriptome of the adult female malaria mosquito vector Anopheles albimanus. BMC Genomics, 207. https://doi.org/10.1186/1471-2164-13-207

Transcriptome of the adult female malaria mosquito vector Anopheles albimanus. / Martinez-Barnetche, Jesus; Gómez-Barreto, Rosa E.; Ovilla-Muñoz, Marbella; Téllez-Sosa, Juan; García-López, David E.; Dinglasan, Rhoel R.; Ubaida Mohien, Ceereena; MacCallum, Robert M.; Redmond, Seth N.; Gibbons, John G.; Rokas, Antonis; Machado, Carlos M.; Cazares-Raga, Febe; González-Cerón, Lilia; Hernández-Martínez, Salvador; Rodríguez-Lopez, Mario H.

In: BMC Genomics, 30.05.2012, p. 207.

Research output: Contribution to journalArticle

Martinez-Barnetche, J, Gómez-Barreto, RE, Ovilla-Muñoz, M, Téllez-Sosa, J, García-López, DE, Dinglasan, RR, Ubaida Mohien, C, MacCallum, RM, Redmond, SN, Gibbons, JG, Rokas, A, Machado, CM, Cazares-Raga, F, González-Cerón, L, Hernández-Martínez, S & Rodríguez-Lopez, MH 2012, 'Transcriptome of the adult female malaria mosquito vector Anopheles albimanus', BMC Genomics, pp. 207. https://doi.org/10.1186/1471-2164-13-207
Martinez-Barnetche J, Gómez-Barreto RE, Ovilla-Muñoz M, Téllez-Sosa J, García-López DE, Dinglasan RR et al. Transcriptome of the adult female malaria mosquito vector Anopheles albimanus. BMC Genomics. 2012 May 30;207. https://doi.org/10.1186/1471-2164-13-207
Martinez-Barnetche, Jesus ; Gómez-Barreto, Rosa E. ; Ovilla-Muñoz, Marbella ; Téllez-Sosa, Juan ; García-López, David E. ; Dinglasan, Rhoel R. ; Ubaida Mohien, Ceereena ; MacCallum, Robert M. ; Redmond, Seth N. ; Gibbons, John G. ; Rokas, Antonis ; Machado, Carlos M. ; Cazares-Raga, Febe ; González-Cerón, Lilia ; Hernández-Martínez, Salvador ; Rodríguez-Lopez, Mario H. / Transcriptome of the adult female malaria mosquito vector Anopheles albimanus. In: BMC Genomics. 2012 ; pp. 207.
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T1 - Transcriptome of the adult female malaria mosquito vector Anopheles albimanus

AU - Martinez-Barnetche, Jesus

AU - Gómez-Barreto, Rosa E.

AU - Ovilla-Muñoz, Marbella

AU - Téllez-Sosa, Juan

AU - García-López, David E.

AU - Dinglasan, Rhoel R.

AU - Ubaida Mohien, Ceereena

AU - MacCallum, Robert M.

AU - Redmond, Seth N.

AU - Gibbons, John G.

AU - Rokas, Antonis

AU - Machado, Carlos M.

AU - Cazares-Raga, Febe

AU - González-Cerón, Lilia

AU - Hernández-Martínez, Salvador

AU - Rodríguez-Lopez, Mario H.

PY - 2012/5/30

Y1 - 2012/5/30

N2 - Background: Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 million years. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex. Results: We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bi-directional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects. Conclusions: We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).

AB - Background: Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 million years. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex. Results: We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bi-directional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects. Conclusions: We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).

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