@article{817d650b589f4e78ae0ff4ccfcaf5766,
title = "Transcriptome Dynamics of Hematopoietic Stem Cell Formation Revealed Using a Combinatorial Runx1 and Ly6a Reporter System",
abstract = "Chen and colleagues generate a Runx1 and Ly6a dual reporter mouse that marks pre-HSCs from specification of hemogenic endothelial cells to their migration to placenta, where they mature and expand, before colonization of the fetal liver as mature HSCs. Single-cell RNA-seq analyses of maturing HSCs reveal progressive acquisition of a quiescent state reminiscent of adult bone marrow long-term repopulating HSCs.",
keywords = "EHT, development, endothelial-to-hematopoietic transition, hematopoietic stem cells, hemogenic endothelial cells, pre-HSCs, reporter, scRNA-seq, transcriptomics",
author = "Chen, {Michael J.} and {Lummertz da Rocha}, Edroaldo and Patrick Cahan and Caroline Kubaczka and Phoebe Hunter and Patricia Sousa and Mullin, {Nathaniel K.} and Yuko Fujiwara and Minh Nguyen and Yuqi Tan and Samuel Landry and Areum Han and Song Yang and Lu, {Yi Fen} and Jha, {Deepak Kumar} and Vo, {Linda T.} and Yi Zhou and North, {Trista E.} and Zon, {Leonard I.} and Daley, {George Q.} and Schlaeger, {Thorsten M.}",
note = "Funding Information: The authors thank Dr. Marella F.T.R. de Bruijn for providing Hsp69EGFP_+23 transgene plasmid, Dr. Michael Davidson for providing the H2B-mKO2 plasmid, Ronald Mathieu and Mahnaz Paktinat for their assistance with FACS, Anthony Hill for his help with confocal microscopy at the IDDRC Cellular Imaging Core at Boston Children's Hospital. We thank Sue-Ping Lee's help at the Imaging Core of Institute of Molecular Biology, Dr. Ming-Zong Lai's support and funding from Academia Sinica , Taiwan, R.O.C. This work was made possible by the generous support of the Boston Children's Hospital Stem Cell Program and the NIDDK ( U54DK110805 ). L.I.Z. is a founder and stockholder of Fate Therapeutics, CAMP4 Therapeutics, Amagma Therapeutics, and Scholar Rock. He is a consultant for Celularity. Funding Information: The authors thank Dr. Marella F.T.R. de Bruijn for providing Hsp69EGFP_+23 transgene plasmid, Dr. Michael Davidson for providing the H2B-mKO2 plasmid, Ronald Mathieu and Mahnaz Paktinat for their assistance with FACS, Anthony Hill for his help with confocal microscopy at the IDDRC Cellular Imaging Core at Boston Children's Hospital. We thank Sue-Ping Lee's help at the Imaging Core of Institute of Molecular Biology, Dr. Ming-Zong Lai's support and funding from Academia Sinica, Taiwan, R.O.C. This work was made possible by the generous support of the Boston Children's Hospital Stem Cell Program and the NIDDK (U54DK110805). L.I.Z. is a founder and stockholder of Fate Therapeutics, CAMP4 Therapeutics, Amagma Therapeutics, and Scholar Rock. He is a consultant for Celularity. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = may,
day = "12",
doi = "10.1016/j.stemcr.2020.03.020",
language = "English (US)",
volume = "14",
pages = "956--971",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "5",
}