TY - JOUR
T1 - Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni
AU - Verjovski-Almeida, Sergio
AU - DeMarco, Ricardo
AU - Martins, Elizabeth A.L.
AU - Guimarães, Pedro E.M.
AU - Ojopi, Elida P.B.
AU - Paquola, Apuã C.M.
AU - Piazza, João P.
AU - Nishiyama, Milton Y.
AU - Kitajima, João P.
AU - Adamson, Rachel E.
AU - Ashton, Peter D.
AU - Bonaldo, Maria F.
AU - Coulson, Patricia S.
AU - Dillon, Gary P.
AU - Farias, Leonardo P.
AU - Gregorio, Sheila P.
AU - Ho, Paulo L.
AU - Leite, Ricardo A.
AU - Malaquias, L. Cosme C.
AU - Marques, Regina C.P.
AU - Miyasato, Patricia A.
AU - Nascimento, Ana L.T.O.
AU - Ohlweiler, Fernanda P.
AU - Reis, Eduardo M.
AU - Ribeiro, Marcela A.
AU - Sá, Renata G.
AU - Stukart, Gaëlle C.
AU - Soares, M. Bento
AU - Gargioni, Cybele
AU - Kawano, Toshie
AU - Rodrigues, Vanderlei
AU - Madeira, Alda M.B.N.
AU - Wilson, R. Alan
AU - Menck, Carlos F.M.
AU - Setubal, João C.
AU - Leite, Luciana C.C.
AU - Dias-Neto, Emmanuel
N1 - Funding Information:
E.D.N. thanks Associação Beneficente Alzira Denise Hertzog da Silva for financial support, D. Rollinson for providing schistosome isolates from Africa and Lebanon and M.G. dos Reis and N. Lucena for providing isolates from northeast Brazil. This project was financed by Fundação de Amparo a Pesquisa do Estado de Sao Paulo and by the Brazilian Ministry of Science and Technology , Conselho Nacional de Desenvolvimento Científico e Tecnológico. The York schistosomiasis group received support from the Biology and Biotechnology Science Research Council, Wellcome Trust and the European Commission Research for Development Programme, Sector Health.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.
AB - Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.
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U2 - 10.1038/ng1237
DO - 10.1038/ng1237
M3 - Article
C2 - 12973350
AN - SCOPUS:0141507054
SN - 1061-4036
VL - 35
SP - 148
EP - 157
JO - Nature genetics
JF - Nature genetics
IS - 2
ER -