Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni

Sergio Verjovski-Almeida; Ricardo DeMarco; Elizabeth A.L. Martins; Pedro E.M. Guimarães; Elida P.B. Ojopi; Apuã C.M. Paquola; João P. Piazza; Milton Y. Nishiyama; João P. Kitajima; Rachel E. Adamson; Peter D. Ashton; Maria F. Bonaldo; Patricia S. Coulson; Gary P. Dillon; Leonardo P. Farias; Sheila P. Gregorio; Paulo L. Ho; Ricardo A. Leite; L. Cosme C. Malaquias; Regina C.P. Marques; Patricia A. Miyasato; Ana L.T.O. Nascimento; Fernanda P. Ohlweiler; Eduardo M. Reis; Marcela A. Ribeiro; Renata G. Sá; Gaëlle C. Stukart; M. Bento Soares; Cybele Gargioni; Toshie Kawano; Vanderlei Rodrigues; Alda M.B.N. Madeira; R. Alan Wilson; Carlos F.M. Menck; João C. Setubal; Luciana C.C. Leite; Emmanuel Dias-Neto

doi:10.1038/ng1237

Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni

Sergio Verjovski-Almeida, Ricardo DeMarco, Elizabeth A.L. Martins, Pedro E.M. Guimarães, Elida P.B. Ojopi, Apuã C.M. Paquola, João P. Piazza, Milton Y. Nishiyama, João P. Kitajima, Rachel E. Adamson, Peter D. Ashton, Maria F. Bonaldo, Patricia S. Coulson, Gary P. Dillon, Leonardo P. Farias, Sheila P. Gregorio, Paulo L. Ho, Ricardo A. Leite, L. Cosme C. Malaquias, Regina C.P. MarquesPatricia A. Miyasato, Ana L.T.O. Nascimento, Fernanda P. Ohlweiler, Eduardo M. Reis, Marcela A. Ribeiro, Renata G. Sá, Gaëlle C. Stukart, M. Bento Soares, Cybele Gargioni, Toshie Kawano, Vanderlei Rodrigues, Alda M.B.N. Madeira, R. Alan Wilson, Carlos F.M. Menck, João C. Setubal, Luciana C.C. Leite, Emmanuel Dias-Neto

Research output: Contribution to journal › Article

Abstract

Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.

Original language	English (US)
Pages (from-to)	148-157
Number of pages	10
Journal	Nature genetics
Volume	35
Issue number	2
DOIs	https://doi.org/10.1038/ng1237
State	Published - Oct 1 2003
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1038/ng1237

Fingerprint Dive into the research topics of 'Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni'. Together they form a unique fingerprint.

Cite this

Verjovski-Almeida, S., DeMarco, R., Martins, E. A. L., Guimarães, P. E. M., Ojopi, E. P. B., Paquola, A. C. M., Piazza, J. P., Nishiyama, M. Y., Kitajima, J. P., Adamson, R. E., Ashton, P. D., Bonaldo, M. F., Coulson, P. S., Dillon, G. P., Farias, L. P., Gregorio, S. P., Ho, P. L., Leite, R. A., Malaquias, L. C. C., ... Dias-Neto, E. (2003). Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. Nature genetics, 35(2), 148-157. https://doi.org/10.1038/ng1237

Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. / Verjovski-Almeida, Sergio; DeMarco, Ricardo; Martins, Elizabeth A.L.; Guimarães, Pedro E.M.; Ojopi, Elida P.B.; Paquola, Apuã C.M.; Piazza, João P.; Nishiyama, Milton Y.; Kitajima, João P.; Adamson, Rachel E.; Ashton, Peter D.; Bonaldo, Maria F.; Coulson, Patricia S.; Dillon, Gary P.; Farias, Leonardo P.; Gregorio, Sheila P.; Ho, Paulo L.; Leite, Ricardo A.; Malaquias, L. Cosme C.; Marques, Regina C.P.; Miyasato, Patricia A.; Nascimento, Ana L.T.O.; Ohlweiler, Fernanda P.; Reis, Eduardo M.; Ribeiro, Marcela A.; Sá, Renata G.; Stukart, Gaëlle C.; Soares, M. Bento; Gargioni, Cybele; Kawano, Toshie; Rodrigues, Vanderlei; Madeira, Alda M.B.N.; Wilson, R. Alan; Menck, Carlos F.M.; Setubal, João C.; Leite, Luciana C.C.; Dias-Neto, Emmanuel.

In: Nature genetics, Vol. 35, No. 2, 01.10.2003, p. 148-157.

Research output: Contribution to journal › Article

Verjovski-Almeida, S, DeMarco, R, Martins, EAL, Guimarães, PEM, Ojopi, EPB, Paquola, ACM, Piazza, JP, Nishiyama, MY, Kitajima, JP, Adamson, RE, Ashton, PD, Bonaldo, MF, Coulson, PS, Dillon, GP, Farias, LP, Gregorio, SP, Ho, PL, Leite, RA, Malaquias, LCC, Marques, RCP, Miyasato, PA, Nascimento, ALTO, Ohlweiler, FP, Reis, EM, Ribeiro, MA, Sá, RG, Stukart, GC, Soares, MB, Gargioni, C, Kawano, T, Rodrigues, V, Madeira, AMBN, Wilson, RA, Menck, CFM, Setubal, JC, Leite, LCC & Dias-Neto, E 2003, 'Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni', Nature genetics, vol. 35, no. 2, pp. 148-157. https://doi.org/10.1038/ng1237

Verjovski-Almeida, Sergio ; DeMarco, Ricardo ; Martins, Elizabeth A.L. ; Guimarães, Pedro E.M. ; Ojopi, Elida P.B. ; Paquola, Apuã C.M. ; Piazza, João P. ; Nishiyama, Milton Y. ; Kitajima, João P. ; Adamson, Rachel E. ; Ashton, Peter D. ; Bonaldo, Maria F. ; Coulson, Patricia S. ; Dillon, Gary P. ; Farias, Leonardo P. ; Gregorio, Sheila P. ; Ho, Paulo L. ; Leite, Ricardo A. ; Malaquias, L. Cosme C. ; Marques, Regina C.P. ; Miyasato, Patricia A. ; Nascimento, Ana L.T.O. ; Ohlweiler, Fernanda P. ; Reis, Eduardo M. ; Ribeiro, Marcela A. ; Sá, Renata G. ; Stukart, Gaëlle C. ; Soares, M. Bento ; Gargioni, Cybele ; Kawano, Toshie ; Rodrigues, Vanderlei ; Madeira, Alda M.B.N. ; Wilson, R. Alan ; Menck, Carlos F.M. ; Setubal, João C. ; Leite, Luciana C.C. ; Dias-Neto, Emmanuel. / Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. In: Nature genetics. 2003 ; Vol. 35, No. 2. pp. 148-157.

@article{84c1460b36ef4558b5f9520c630daf3a,

title = "Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni",

abstract = "Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.",

author = "Sergio Verjovski-Almeida and Ricardo DeMarco and Martins, {Elizabeth A.L.} and Guimar{\~a}es, {Pedro E.M.} and Ojopi, {Elida P.B.} and Paquola, {Apu{\~a} C.M.} and Piazza, {Jo{\~a}o P.} and Nishiyama, {Milton Y.} and Kitajima, {Jo{\~a}o P.} and Adamson, {Rachel E.} and Ashton, {Peter D.} and Bonaldo, {Maria F.} and Coulson, {Patricia S.} and Dillon, {Gary P.} and Farias, {Leonardo P.} and Gregorio, {Sheila P.} and Ho, {Paulo L.} and Leite, {Ricardo A.} and Malaquias, {L. Cosme C.} and Marques, {Regina C.P.} and Miyasato, {Patricia A.} and Nascimento, {Ana L.T.O.} and Ohlweiler, {Fernanda P.} and Reis, {Eduardo M.} and Ribeiro, {Marcela A.} and S{\'a}, {Renata G.} and Stukart, {Ga{\"e}lle C.} and Soares, {M. Bento} and Cybele Gargioni and Toshie Kawano and Vanderlei Rodrigues and Madeira, {Alda M.B.N.} and Wilson, {R. Alan} and Menck, {Carlos F.M.} and Setubal, {Jo{\~a}o C.} and Leite, {Luciana C.C.} and Emmanuel Dias-Neto",

year = "2003",

month = oct,

day = "1",

doi = "10.1038/ng1237",

language = "English (US)",

volume = "35",

pages = "148--157",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni

AU - Verjovski-Almeida, Sergio

AU - DeMarco, Ricardo

AU - Martins, Elizabeth A.L.

AU - Guimarães, Pedro E.M.

AU - Ojopi, Elida P.B.

AU - Paquola, Apuã C.M.

AU - Piazza, João P.

AU - Nishiyama, Milton Y.

AU - Kitajima, João P.

AU - Adamson, Rachel E.

AU - Ashton, Peter D.

AU - Bonaldo, Maria F.

AU - Coulson, Patricia S.

AU - Dillon, Gary P.

AU - Farias, Leonardo P.

AU - Gregorio, Sheila P.

AU - Ho, Paulo L.

AU - Leite, Ricardo A.

AU - Malaquias, L. Cosme C.

AU - Marques, Regina C.P.

AU - Miyasato, Patricia A.

AU - Nascimento, Ana L.T.O.

AU - Ohlweiler, Fernanda P.

AU - Reis, Eduardo M.

AU - Ribeiro, Marcela A.

AU - Sá, Renata G.

AU - Stukart, Gaëlle C.

AU - Soares, M. Bento

AU - Gargioni, Cybele

AU - Kawano, Toshie

AU - Rodrigues, Vanderlei

AU - Madeira, Alda M.B.N.

AU - Wilson, R. Alan

AU - Menck, Carlos F.M.

AU - Setubal, João C.

AU - Leite, Luciana C.C.

AU - Dias-Neto, Emmanuel

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.

AB - Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.

UR - http://www.scopus.com/inward/record.url?scp=0141507054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141507054&partnerID=8YFLogxK

U2 - 10.1038/ng1237

DO - 10.1038/ng1237

M3 - Article

C2 - 12973350

AN - SCOPUS:0141507054

VL - 35

SP - 148

EP - 157

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 2

ER -