TY - JOUR
T1 - Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions
AU - Xu, Xiaojin
AU - Dong, Yuemei
AU - Abraham, Eappen G.
AU - Kocan, Anna
AU - Srinivasan, Prakash
AU - Ghosh, Anil K.
AU - Sinden, Robert E.
AU - Ribeiro, Jose M.C.
AU - Jacobs-Lorena, Marcelo
AU - Kafatos, Fotis C.
AU - Dimopoulos, George
N1 - Funding Information:
We would like to thank the EMBL microarray facility for assistance with microarray construction, Ms. C. Hsieu for help with data processing and Dr. N. Mohammed for the CMMI microarray facility management. This work has been supported by funding from the European Commission Research Training Networks Grants HPRN-CT-2000-00080, grants from the National Institute of Allergy and Infectious Diseases, the Imperial College London and The Johns Hopkins Malaria Research Institute, Ellison Medical Foundation and the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR).
PY - 2005/7
Y1 - 2005/7
N2 - Simultaneous microarray-based transcription analysis of 4987 Anopheles stephensi midgut and Plasmodium berghei infection stage specific cDNAs was done at seven successive time points: 6, 20 and 40 h, and 4, 8, 14 and 20 days after ingestion of malaria infected blood. The study reveals the molecular components of several Anopheles processes relating to blood digestion, midgut expansion and response to Plasmodium-infected blood such as digestive enzymes, transporters, cytoskeletal and structural components and stress and immune responsive factors. In parallel, the analysis provide detailed expression patterns of Plasmodium genes encoding essential developmental and metabolic factors and proteins implicated in interaction with the mosquito vector and vertebrate host such as kinases, transcription and translational factors, cytoskeletal components and a variety of surface proteins, some of which are potent vaccine targets. Temporal correlation between transcription profiles of both organisms identifies putative gene clusters of interacting processes, such as Plasmodium invasion of the midgut epithelium, Anopheles immune responses to Plasmodium infection, and apoptosis and expulsion of invaded midgut cells from the epithelium. Intriguing transcription patterns for highly variable Plasmodium surface antigens may indicate parasite strategies to avoid recognition by the mosquito's immune surveillance system.
AB - Simultaneous microarray-based transcription analysis of 4987 Anopheles stephensi midgut and Plasmodium berghei infection stage specific cDNAs was done at seven successive time points: 6, 20 and 40 h, and 4, 8, 14 and 20 days after ingestion of malaria infected blood. The study reveals the molecular components of several Anopheles processes relating to blood digestion, midgut expansion and response to Plasmodium-infected blood such as digestive enzymes, transporters, cytoskeletal and structural components and stress and immune responsive factors. In parallel, the analysis provide detailed expression patterns of Plasmodium genes encoding essential developmental and metabolic factors and proteins implicated in interaction with the mosquito vector and vertebrate host such as kinases, transcription and translational factors, cytoskeletal components and a variety of surface proteins, some of which are potent vaccine targets. Temporal correlation between transcription profiles of both organisms identifies putative gene clusters of interacting processes, such as Plasmodium invasion of the midgut epithelium, Anopheles immune responses to Plasmodium infection, and apoptosis and expulsion of invaded midgut cells from the epithelium. Intriguing transcription patterns for highly variable Plasmodium surface antigens may indicate parasite strategies to avoid recognition by the mosquito's immune surveillance system.
KW - Anopheles
KW - Gene expression
KW - Plasmodium
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U2 - 10.1016/j.molbiopara.2005.02.013
DO - 10.1016/j.molbiopara.2005.02.013
M3 - Article
C2 - 15907562
AN - SCOPUS:21144445956
SN - 0166-6851
VL - 142
SP - 76
EP - 87
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -