Transcriptional target-based expression cloning of immunoregulatory molecules

Rebecca L. Lamason, Stefanie M. Lew, Joel L Pomerantz

Research output: Contribution to journalArticle

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

Original languageEnglish (US)
Pages (from-to)172-178
Number of pages7
JournalImmunologic Research
Volume47
Issue number1-3
DOIs
StatePublished - Jul 2010

Fingerprint

Organism Cloning
Cytokine Receptors
Transcription Factors
Complementary DNA
Gene Expression
Research
Genes
Proteins

Keywords

  • cDNA library
  • Expression-cloning
  • Gene discovery
  • HIV-1
  • NF-κB

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Transcriptional target-based expression cloning of immunoregulatory molecules. / Lamason, Rebecca L.; Lew, Stefanie M.; Pomerantz, Joel L.

In: Immunologic Research, Vol. 47, No. 1-3, 07.2010, p. 172-178.

Research output: Contribution to journalArticle

Lamason, Rebecca L. ; Lew, Stefanie M. ; Pomerantz, Joel L. / Transcriptional target-based expression cloning of immunoregulatory molecules. In: Immunologic Research. 2010 ; Vol. 47, No. 1-3. pp. 172-178.
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