Transcriptional target-based expression cloning of immunoregulatory molecules

Rebecca L. Lamason, Stefanie M. Lew, Joel L. Pomerantz

Research output: Contribution to journalReview article

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

Original languageEnglish (US)
Pages (from-to)172-178
Number of pages7
JournalImmunologic Research
Volume47
Issue number1-3
DOIs
StatePublished - Jul 1 2010

Keywords

  • Expression-cloning
  • Gene discovery
  • HIV-1
  • NF-κB
  • cDNA library

ASJC Scopus subject areas

  • Immunology

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