Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1

Ceri Batchelder, Melanie A. Dunn, Bob Choy, Yong Suh, Conrad Cassie, Eun Yong Shim, Tae Ho Shin, Craig Mello, Geraldine Seydoux, T. Keith Blackwell

Research output: Contribution to journalArticle

Abstract

In the early Caenorhabditis elegans embryo, maternally expressed PIE-1 protein is required in germ-line blastomeres to inhibit somatic differentiation, maintain an absence of mRNA transcription, and block phosphorylation of the RNA polymerase II large subunit (Pol II) carboxy- terminal domain (CTD). We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays. By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly. A sequence element that is required for this repressor activity is similar to the Pol II CTD heptapeptide repeat, suggesting that the PIE-1 repression domain might target a protein complex that can bind the CTD. An alteration of this sequence element that blocks repression also impairs the ability of a transgene to rescue a pie-1 mutation, suggesting that this repressor activity may be important for PIE-1 function in vivo.

Original languageEnglish (US)
Pages (from-to)202-212
Number of pages11
JournalGenes & development
Volume13
Issue number2
StatePublished - Jan 15 1999

Fingerprint

Caenorhabditis elegans
Germ Cells
Blastomeres
Transgenes
Proteins
Embryonic Structures
Cell Culture Techniques
Yeasts
Phosphorylation
Messenger RNA
Mutation
DNA
RNA polymerase II largest subunit

Keywords

  • C. elegans
  • Germ line
  • Repression
  • RNA Pol II CTD
  • Transcription
  • Zinc finger

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Batchelder, C., Dunn, M. A., Choy, B., Suh, Y., Cassie, C., Shim, E. Y., ... Blackwell, T. K. (1999). Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. Genes & development, 13(2), 202-212.

Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. / Batchelder, Ceri; Dunn, Melanie A.; Choy, Bob; Suh, Yong; Cassie, Conrad; Shim, Eun Yong; Shin, Tae Ho; Mello, Craig; Seydoux, Geraldine; Blackwell, T. Keith.

In: Genes & development, Vol. 13, No. 2, 15.01.1999, p. 202-212.

Research output: Contribution to journalArticle

Batchelder, C, Dunn, MA, Choy, B, Suh, Y, Cassie, C, Shim, EY, Shin, TH, Mello, C, Seydoux, G & Blackwell, TK 1999, 'Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1', Genes & development, vol. 13, no. 2, pp. 202-212.
Batchelder C, Dunn MA, Choy B, Suh Y, Cassie C, Shim EY et al. Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. Genes & development. 1999 Jan 15;13(2):202-212.
Batchelder, Ceri ; Dunn, Melanie A. ; Choy, Bob ; Suh, Yong ; Cassie, Conrad ; Shim, Eun Yong ; Shin, Tae Ho ; Mello, Craig ; Seydoux, Geraldine ; Blackwell, T. Keith. / Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. In: Genes & development. 1999 ; Vol. 13, No. 2. pp. 202-212.
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