Transcriptional regulation of the gene for the second component of human complement: Promoter analysis

Kathleen E. Sullivan, David Valle, Jerry A. Winkelstein, Lai‐Chu ‐C Wu, R. Duncan Campbell

Research output: Contribution to journalArticlepeer-review

Abstract

The 5′ flanking region of the human gene for the second component of complement was sequenced and analyzed functionally. RNase protection demonstrated a cluster of four initiation sites in the 5′ flanking region utilized in the hepatoma cell line, HepG2. Utilization of all four initiation sites increased in response to γ‐interferon (IFN‐γ). Transient transfection analysis was used to examine cis‐acting sequence motifs controlling transcription from the 5′‐flanking region. We identified a 228‐bp minimal promoter fragment which was able to direct basal and IFN‐γ inducible transcription from authentic initiation sites. Sequence motifs outside of this region may modulate the transcriptional regulation of the second component of complement. Although complement components are not coordinately regulated, we identified four regions of significant homology with the promoters of multiple other complement components. Three of these regions were within the minimal promoter fragment.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalEuropean Journal of Immunology
Volume24
Issue number2
DOIs
StatePublished - Feb 1994

Keywords

  • C2
  • Complement
  • Transcription

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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