Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation

Mingming Zhao, Jaimy Joy, Weiqiang Zhou, Supriyo De, William H. Wood, Kevin G. Becker, Hongkai Ji, Ranjan Sen

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns in activated B lymphoma cells. By combining RELA binding, RNA polymerase II (Pol II) recruitment, and perturbation of NF-κB activation, we demonstrate that kinetic differences amongst early- and late-activated RELA target genes can be understood based on chromatin configuration prior to cell activation and RELA-dependent priming, respectively. We also identified genes that were repressed by RELA activation and others that responded to RELA-activated transcription factors. Cumulatively, our studies define an NF-κB-responsive inducible gene cascade in activated B cells.

Original languageEnglish (US)
Article numbere2006347
JournalPLoS biology
Volume16
Issue number9
DOIs
StatePublished - Sep 2018

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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