@article{313b02755e5540a4b907bf5ac26eb400,
title = "Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors",
abstract = "Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny (online database can be accessed in http://www. myogenesis.net/).",
author = "Choi, {In Young} and Hotae Lim and Cho, {Hyeon Jin} and Yohan Oh and Chou, {Bin Kuan} and Hao Bai and Linzhao Cheng and Kim, {Yong Jun} and Sanghwan Hyun and Hyesoo Kim and Shin, {Joo Heon} and Gabsang Lee",
note = "Funding Information: This work was supported by grants from the National Institutes of Health through R01NS093213, R01AR070751 (GL), the Maryland Stem Cell Research Funding (MSCRF; GL), the Muscular Dystrophy Association (MDA: GL), the FSH Society (GL), and the Global Research Development Center program from the Korea National Research Foundation (GL, S-H H). The authors acknowledge the Cytogenetic Core Facility (supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health under Award Number U54HD079123). We thank the Developmental Studies Hybridoma Bank for antibodies. National Institute of Arthritis and Musculoskeletal and Skin Diseases R01AR070751 Gabsang Lee. Maryland Stem Cell Research Fund 2017-MSCRFD-3941 Gabsang Lee. National Institutes of Health R01NS093213 Gabsang Lee. Maryland Stem Cell Research Fund 2017-MSCRFD-3941 Gabsang Lee. Muscular Dystrophy Association 381465 Gabsang Lee. FSH Society FSHS-82014-03 Gabsang Lee. National Research Foundation of Korea 2017K1A4A3014959 SangHwan Hyun Gabsang Lee. Funding Information: This work was supported by grants from the National Institutes of Health through R01NS093213, R01AR070751 (GL), the Maryland Stem Cell Research Funding (MSCRF; GL), the Muscular Dystrophy Association (MDA: GL), the FSH Society (GL), and the Global Research Development Center program from the Korea National Research Foundation (GL, S-H H). The authors acknowledge the Cytogenetic Core Facility (supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health under Award Number U54HD079123). We thank the Developmental Studies Hybridoma Bank for antibodies. Publisher Copyright: {\textcopyright} Choi et al.",
year = "2020",
month = feb,
doi = "10.7554/eLife.46981",
language = "English (US)",
volume = "9",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}