Transcriptional analysis of the adenovirus-5 EIII promoter

Absence of sequence specificity for stimulation by EIa gene products

Todd Leff, Jeffry Lynn Corden, René Elkaim, Paolo Sassone-Corsi

Research output: Contribution to journalArticle

Abstract

To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.

Original languageEnglish (US)
Pages (from-to)1209-1221
Number of pages13
JournalNucleic Acids Research
Volume13
Issue number4
DOIs
StatePublished - Feb 25 1985
Externally publishedYes

Fingerprint

Transcription
HeLa Cells
Promoter
Adenoviridae
Specificity
Genes
Gene
Cell Extracts
Transfection
Deletion
Cell
Mutant
Series
In Vitro Techniques

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Transcriptional analysis of the adenovirus-5 EIII promoter : Absence of sequence specificity for stimulation by EIa gene products. / Leff, Todd; Corden, Jeffry Lynn; Elkaim, René; Sassone-Corsi, Paolo.

In: Nucleic Acids Research, Vol. 13, No. 4, 25.02.1985, p. 1209-1221.

Research output: Contribution to journalArticle

@article{8a7cf0428e2e4e16b36d526cbe4bb1d1,
title = "Transcriptional analysis of the adenovirus-5 EIII promoter: Absence of sequence specificity for stimulation by EIa gene products",
abstract = "To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.",
author = "Todd Leff and Corden, {Jeffry Lynn} and Ren{\'e} Elkaim and Paolo Sassone-Corsi",
year = "1985",
month = "2",
day = "25",
doi = "10.1093/nar/13.4.1209",
language = "English (US)",
volume = "13",
pages = "1209--1221",
journal = "Nucleic Acids Research",
issn = "1362-4962",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Transcriptional analysis of the adenovirus-5 EIII promoter

T2 - Absence of sequence specificity for stimulation by EIa gene products

AU - Leff, Todd

AU - Corden, Jeffry Lynn

AU - Elkaim, René

AU - Sassone-Corsi, Paolo

PY - 1985/2/25

Y1 - 1985/2/25

N2 - To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.

AB - To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at -55/-57, and an additional element located between -111 and -233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between -15 and -233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.

UR - http://www.scopus.com/inward/record.url?scp=0022431869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022431869&partnerID=8YFLogxK

U2 - 10.1093/nar/13.4.1209

DO - 10.1093/nar/13.4.1209

M3 - Article

VL - 13

SP - 1209

EP - 1221

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 1362-4962

IS - 4

ER -