Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro

Research output: Contribution to journalArticle

Abstract

SV40 DNA replication in vivo is greatly stimulated by cis-acting transcriptional elements. We studied a model viral chromosome containing a single binding site for the cellular transcriptional activator, nuclear factor I ( NF-I CTF), located adjacent to the replication origin. The presence of the NF-I recognition site increased replication efficiency over 20-fold in vivo. Purified NF-I had little effect on the replication efficiency in the standard SV40 cell-free system when the template was introduced as naked DNA. However, NF-I specifically prevented the repression of DNA replication that occurred when the template was preassembled into chromatin. Our data support a model in which the binding of a transcriptional activator perturbs the local distribution of nucleosomes, thereby increasing the accessibility of the origin region.

Original languageEnglish (US)
Pages (from-to)541-551
Number of pages11
JournalCell
Volume59
Issue number3
DOIs
StatePublished - Nov 3 1989

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NFI Transcription Factors
DNA Replication
Replication Origin
Cell-Free System
Nucleosomes
DNA
Chromatin
Chromosomes
Binding Sites
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro. / Cheng, Linzhao; Kelly, Thomas.

In: Cell, Vol. 59, No. 3, 03.11.1989, p. 541-551.

Research output: Contribution to journalArticle

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