Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I

John Tower, Barbara Sollner-Webb

Research output: Contribution to journalArticle

Abstract

We have identified the species-nonspecific factor required for mouse rDNA transcription, factor C, as an activated subform of RNA polymerase I. C is an RNA polymerase I since it copurifies with bulk polymerase I activity on the three chromatographic columns used to achieve a virtually homogenous preparation of polymerase I, as well as on four additional matrices; it is quantitatively neutralized as well as immunoprecipitated by two different types of anti-polymerase I antibodies; and it has thermal lability identical to that of bulk polymerase I. However, C is clearly distinct from bulk polymerase I in its ability to participate in the stable rDNA transcription complex and to catalyze accurate initiation of rRNA synthesis. It also has a greater sedimentation coefficient than bulk polymerase I. Furthermore, this activated polymerase subform is specifically lacking in extracts of cells in which rDNA transcription was down-regulated because of cycloheximide treatment or attainment of stationary phase. These data suggest that regulation of rDNA transcription in vivo may involve modulation in availability of the activated polymerase I subform.

Original languageEnglish (US)
Pages (from-to)873-883
Number of pages11
JournalCell
Volume50
Issue number6
DOIs
StatePublished - Sep 11 1987

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RNA Polymerase I
Transcription
Ribosomal DNA
RNA Polymerase III
Cycloheximide
Cell Extracts
Sedimentation
Transcription Factors
Hot Temperature
Modulation
Availability
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I. / Tower, John; Sollner-Webb, Barbara.

In: Cell, Vol. 50, No. 6, 11.09.1987, p. 873-883.

Research output: Contribution to journalArticle

Tower, John ; Sollner-Webb, Barbara. / Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I. In: Cell. 1987 ; Vol. 50, No. 6. pp. 873-883.
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