Transcription factor YY1 promotes adipogenesis via inhibiting CHOP-10 expression

Hai Yan Huang, Xi Li, Mei Liu, Tan Jing Song, Qun He, Chun Gu Ma, Qi Qun Tang

Research output: Contribution to journalArticlepeer-review

Abstract

CHOP-10, a dominant-negative member of the C/EBP family of transcription factors, is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ through heterodimerization with its leucine zipper during 3T3-L1 preadipocyte differentiation. Our previous studies indicated that, FBS leads to the down-regulation of CHOP-10 expression after induction, and releasing C/EBPβ from inhibitory constraint, allowing the transactivation of C/EBPα and PPARγ genes, transcription factors required for terminal adipocyte differentiation. In the present study, we reported that FBS induced the expression of YY1, which bound to CHOP-10 promoter via two adjacent YY1-binding sites, suppressing its expression. The knock-down of YY1 expression with YY1 siRNA increased the expression of CHOP-10, inhibiting adipocyte differentiation. IGF-1, a growth factor present in greater concentration in FBS, independently induced the expression of YY1, and contributed to the down-regulation of CHOP-10 during the adipocyte differentiation program. Our studies suggested that YY1 can be a new adipocyte differentiation stimulator.

Original languageEnglish (US)
Pages (from-to)496-500
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume375
Issue number4
DOIs
StatePublished - Oct 31 2008
Externally publishedYes

Keywords

  • Adipocyte differentiation
  • CHOP-10
  • FBS
  • IGF-1
  • YY1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Transcription factor YY1 promotes adipogenesis via inhibiting CHOP-10 expression'. Together they form a unique fingerprint.

Cite this