TY - JOUR
T1 - Transcription factor YY1 promotes adipogenesis via inhibiting CHOP-10 expression
AU - Huang, Hai Yan
AU - Li, Xi
AU - Liu, Mei
AU - Song, Tan Jing
AU - He, Qun
AU - Ma, Chun Gu
AU - Tang, Qi Qun
PY - 2008/10/31
Y1 - 2008/10/31
N2 - CHOP-10, a dominant-negative member of the C/EBP family of transcription factors, is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ through heterodimerization with its leucine zipper during 3T3-L1 preadipocyte differentiation. Our previous studies indicated that, FBS leads to the down-regulation of CHOP-10 expression after induction, and releasing C/EBPβ from inhibitory constraint, allowing the transactivation of C/EBPα and PPARγ genes, transcription factors required for terminal adipocyte differentiation. In the present study, we reported that FBS induced the expression of YY1, which bound to CHOP-10 promoter via two adjacent YY1-binding sites, suppressing its expression. The knock-down of YY1 expression with YY1 siRNA increased the expression of CHOP-10, inhibiting adipocyte differentiation. IGF-1, a growth factor present in greater concentration in FBS, independently induced the expression of YY1, and contributed to the down-regulation of CHOP-10 during the adipocyte differentiation program. Our studies suggested that YY1 can be a new adipocyte differentiation stimulator.
AB - CHOP-10, a dominant-negative member of the C/EBP family of transcription factors, is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ through heterodimerization with its leucine zipper during 3T3-L1 preadipocyte differentiation. Our previous studies indicated that, FBS leads to the down-regulation of CHOP-10 expression after induction, and releasing C/EBPβ from inhibitory constraint, allowing the transactivation of C/EBPα and PPARγ genes, transcription factors required for terminal adipocyte differentiation. In the present study, we reported that FBS induced the expression of YY1, which bound to CHOP-10 promoter via two adjacent YY1-binding sites, suppressing its expression. The knock-down of YY1 expression with YY1 siRNA increased the expression of CHOP-10, inhibiting adipocyte differentiation. IGF-1, a growth factor present in greater concentration in FBS, independently induced the expression of YY1, and contributed to the down-regulation of CHOP-10 during the adipocyte differentiation program. Our studies suggested that YY1 can be a new adipocyte differentiation stimulator.
KW - Adipocyte differentiation
KW - CHOP-10
KW - FBS
KW - IGF-1
KW - YY1
UR - http://www.scopus.com/inward/record.url?scp=51449083799&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51449083799&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.07.151
DO - 10.1016/j.bbrc.2008.07.151
M3 - Article
C2 - 18694725
AN - SCOPUS:51449083799
SN - 0006-291X
VL - 375
SP - 496
EP - 500
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -