Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes

J. Imbert, M. Zafarullah, V. Cizewski Culotta, L. Gedamu, D. Hamer

Research output: Contribution to journalArticle

Abstract

Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and purified to near homogeneity a 74-kilodalton mouse nuclear protein that specifically binds to certain MRE sequences. This protein, MBF-I, was employing as an affinity reagent a trout MRE that is shown to be functional in mouse cells but which lacks the G+C-rich and SP1-like sequences found in many mammalian MT gene promoters. Using point-mutated MREs, we showed that there is a strong correlation between DNA binding in vitro and MT gene regulation in vivo, suggesting a direct role of MBF-I in MT gene transcription. We also showed that MBF-I can induce MT gene transcription in vitro in a mouse extract and that this stimulation requires zinc.

Original languageEnglish (US)
Pages (from-to)5315-5323
Number of pages9
JournalMolecular and cellular biology
Volume9
Issue number12
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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