Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region of the bovine αs1 casein gene in the mammary gland of transgenic mice and in mouse embryonic stem cells

E. S. Zakharova, Marina Pryzhkova, A. V. Kibardin, T. G. Ermolkevich, S. G. Kadulin, N. V. Gnuchev, S. L. Kiselev

Research output: Contribution to journalArticle

Abstract

The regulatory region of the bovine αS1 casein gene was used to obtain two genetic constructs for expression of human lactoferrin in the mammary gland of transgenic animals. Several transfected mouse embryonic stem cell (ESC) lines and primary transgenic mice were generated with these constructs. Recombinant lactoferrin was not detected in milk of transgenic mice by Western blotting. However, a recombinant transcript was found in RNAs isolated from mammary glands of transgenic females during lactation and from transfected ESC lines.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalRussian Journal of Genetics
Volume41
Issue number3
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

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Lactoferrin
Nucleic Acid Regulatory Sequences
Human Mammary Glands
Caseins
Transgenic Mice
Cell Line
Messenger RNA
Genetically Modified Animals
Embryonic Stem Cells
Lactation
Genes
Milk
Western Blotting
RNA
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Genetics

Cite this

Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region of the bovine αs1 casein gene in the mammary gland of transgenic mice and in mouse embryonic stem cells. / Zakharova, E. S.; Pryzhkova, Marina; Kibardin, A. V.; Ermolkevich, T. G.; Kadulin, S. G.; Gnuchev, N. V.; Kiselev, S. L.

In: Russian Journal of Genetics, Vol. 41, No. 3, 01.03.2005, p. 227-232.

Research output: Contribution to journalArticle

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AU - Gnuchev, N. V.

AU - Kiselev, S. L.

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