Transcript profile of cellular senescence-related genes in Fuchs endothelial corneal dystrophy

Mario Matthaei, Angela Y. Zhu, Laura Kallay, Charles G Eberhart, Claus Cursiefen, Albert Jun

Research output: Contribution to journalArticle

Abstract

Fuchs endothelial corneal dystrophy (FECD) is a genetically heterogeneous disease. Hypothesizing that cellular senescence may be relevant in FECD pathogenesis, genetically undifferentiated late-onset FECD endothelial samples were analyzed to identify common changes of specific senescence-related transcripts. Total RNA was extracted from 21 FECD endothelial samples retrieved from patients undergoing lamellar keratoplasty due to clinically diagnosed end-stage FECD and from 12 endothelial samples retrieved from normal autopsy eyes. Taqman low density array (TLDA) cards were used to analyze differential expression of 89 cellular senescence-related transcripts. Result validation was performed using individual real-time PCR assays. TLDA-analysis demonstrated differential expression of 31 transcripts (fold-change >1.5; p

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalExperimental Eye Research
Volume129
DOIs
StatePublished - Oct 1 2014

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Fuchs' Endothelial Dystrophy
Cell Aging
Genes
Corneal Transplantation
Real-Time Polymerase Chain Reaction
Autopsy
RNA

Keywords

  • CDKN2A
  • Cellular senescence
  • Corneal endothelium
  • Fuchs endothelial corneal dystrophy
  • NOX4
  • Oxidative stress
  • Pathogenesis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Transcript profile of cellular senescence-related genes in Fuchs endothelial corneal dystrophy. / Matthaei, Mario; Zhu, Angela Y.; Kallay, Laura; Eberhart, Charles G; Cursiefen, Claus; Jun, Albert.

In: Experimental Eye Research, Vol. 129, 01.10.2014, p. 13-17.

Research output: Contribution to journalArticle

Matthaei, Mario ; Zhu, Angela Y. ; Kallay, Laura ; Eberhart, Charles G ; Cursiefen, Claus ; Jun, Albert. / Transcript profile of cellular senescence-related genes in Fuchs endothelial corneal dystrophy. In: Experimental Eye Research. 2014 ; Vol. 129. pp. 13-17.
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