Transcranial Recording of Electrophysiological Neural Activity in the Rodent Brain in vivo Using Functional Photoacoustic Imaging of Near-Infrared Voltage-Sensitive Dye

Jeeun Kang, Haichong K. Zhang, Shilpa D. Kadam, Julie Fedorko, Heather Valentine, Adarsha P. Malla, Ping Yan, Maged Harraz, Jin U. Kang, Arman Rahmim, Albert Gjedde, Leslie M. Loew, Dean Foster Wong, Emad Boctor

Research output: Contribution to journalArticle

Abstract

Minimally-invasive monitoring of electrophysiological neural activities in real-time—that enables quantification of neural functions without a need for invasive craniotomy and the longer time constants of fMRI and PET—presents a very challenging yet significant task for neuroimaging. In this paper, we present in vivo functional PA (fPA) imaging of chemoconvulsant rat seizure model with intact scalp using a fluorescence quenching-based cyanine voltage-sensitive dye (VSD) characterized by a lipid vesicle model mimicking different levels of membrane potential variation. The framework also involves use of a near-infrared VSD delivered through the blood-brain barrier (BBB), opened by pharmacological modulation of adenosine receptor signaling. Our normalized time-frequency analysis presented in vivo VSD response in the seizure group significantly distinguishable from those of the control groups at sub-mm spatial resolution. Electroencephalogram (EEG) recording confirmed the changes of severity and frequency of brain activities, induced by chemoconvulsant seizures of the rat brain. The findings demonstrate that the near-infrared fPA VSD imaging is a promising tool for in vivo recording of brain activities through intact scalp, which would pave a way to its future translation in real time human brain imaging.

Original languageEnglish (US)
Article number579
JournalFrontiers in Neuroscience
Volume13
DOIs
StatePublished - Aug 9 2019

Fingerprint

Rodentia
Seizures
Coloring Agents
Scalp
Neuroimaging
Voltage-Sensitive Dye Imaging
Brain
Purinergic P1 Receptors
Craniotomy
Blood-Brain Barrier
Membrane Potentials
Electroencephalography
Fluorescence
Magnetic Resonance Imaging
Pharmacology
Lipids
Control Groups

Keywords

  • near-infrared
  • neuroimaging
  • photoacoustic
  • seizure
  • transcranial
  • voltage-sensitive dye

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transcranial Recording of Electrophysiological Neural Activity in the Rodent Brain in vivo Using Functional Photoacoustic Imaging of Near-Infrared Voltage-Sensitive Dye. / Kang, Jeeun; Zhang, Haichong K.; Kadam, Shilpa D.; Fedorko, Julie; Valentine, Heather; Malla, Adarsha P.; Yan, Ping; Harraz, Maged; Kang, Jin U.; Rahmim, Arman; Gjedde, Albert; Loew, Leslie M.; Wong, Dean Foster; Boctor, Emad.

In: Frontiers in Neuroscience, Vol. 13, 579, 09.08.2019.

Research output: Contribution to journalArticle

Kang, Jeeun ; Zhang, Haichong K. ; Kadam, Shilpa D. ; Fedorko, Julie ; Valentine, Heather ; Malla, Adarsha P. ; Yan, Ping ; Harraz, Maged ; Kang, Jin U. ; Rahmim, Arman ; Gjedde, Albert ; Loew, Leslie M. ; Wong, Dean Foster ; Boctor, Emad. / Transcranial Recording of Electrophysiological Neural Activity in the Rodent Brain in vivo Using Functional Photoacoustic Imaging of Near-Infrared Voltage-Sensitive Dye. In: Frontiers in Neuroscience. 2019 ; Vol. 13.
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