Transactivation and inhibitory domains of Hypoxia-inducible factor 1α

Modulation of transcriptional activity by oxygen tension

Bing Hua Jiang, Jenny Z. Zheng, Sandra W. Leung, Rick Roe, Gregg L Semenza

Research output: Contribution to journalArticle

Abstract

Hypoxia-inducible factor 1 (HIF-1) binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in hypoxic cells. HIF-1 is a basic helix-loop-helix transcription factor composed of HIF-1α and HIF-1β subunits. Here, we demonstrate that HIF-1α contains two transactivation domains located between amino acids 531 and 826. When expressed as GAL4 fusion proteins, the transcriptional activity of these domains increased in response to hypoxia. Fusion protein levels were unaffected by changes in cellular O2 tension. Two minimal transactivation domains were localized to amino acid residues 531-575 and 786-826. The transcriptional activation domains were separated by amino acid sequences that inhibited transactivation. Deletion analysis demonstrated that the gradual removal of inhibitory domain sequences (amino acids 576785) was associated with progressively increased transcriptional activity of the fusion proteins, especially in cells cultured at 20% O2. Transcriptional activity of GAL4/HIF-1α fusion proteins was increased in cells exposed to 1% O2, cobalt chloride, or desferrioxamine, each of which also increased levels of endogenous HIF-lα protein but did not affect fusion protein levels. These results indicate that increased transcriptional activity mediated by HIF-1 in hypoxic cells results from both increased HIF-1α protein levels and increased activity of HIF-1α transactivation domains.

Original languageEnglish (US)
Pages (from-to)19253-19260
Number of pages8
JournalJournal of Biological Chemistry
Volume272
Issue number31
DOIs
StatePublished - Aug 1 1997

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Hypoxia-Inducible Factor 1
Transcriptional Activation
Modulation
Oxygen
Fusion reactions
Proteins
Amino Acids
Amino Acid Sequence
Cells
Basic Helix-Loop-Helix Transcription Factors
Deferoxamine
Response Elements
Transcription
Erythropoietin
Vascular Endothelial Growth Factor A
Cultured Cells
Genes
Chemical activation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transactivation and inhibitory domains of Hypoxia-inducible factor 1α : Modulation of transcriptional activity by oxygen tension. / Jiang, Bing Hua; Zheng, Jenny Z.; Leung, Sandra W.; Roe, Rick; Semenza, Gregg L.

In: Journal of Biological Chemistry, Vol. 272, No. 31, 01.08.1997, p. 19253-19260.

Research output: Contribution to journalArticle

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