Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II

Hiroshi Okamoto, Christian T. Sheline, Jeffry L. Corden, Katherine A. Jones, B. Matija Peterlin

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV)encoded trans-activator (Tat) acts through the transactivation response element RNA stem-loop to increase greatly the processivity of RNA polymerase II. Without Tat, transcription originating from the HIV promoter is attenuated. In this study, we demonstrate that transcriptional activation by Tat in vivo and in vitro requires the C-terminal domain (CTD) of RNA polymerase II. In contrast, the CTD is not required for basal transcription and for the formation of short, attenuated transcripts. Thus, trans-activation by Tat resembles enhancer- dependent activation of transcription. These results suggest that effects of Tat on the processivity of RNA polymerase II require proteins that are associated with the CTD and may result in the phosphorylation of the CTD.

Original languageEnglish (US)
Pages (from-to)11575-11579
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number21
DOIs
StatePublished - Oct 15 1996

ASJC Scopus subject areas

  • General

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