TY - JOUR
T1 - Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II
AU - Okamoto, Hiroshi
AU - Sheline, Christian T.
AU - Corden, Jeffry L.
AU - Jones, Katherine A.
AU - Peterlin, B. Matija
PY - 1996/10/15
Y1 - 1996/10/15
N2 - Human immunodeficiency virus (HIV)encoded trans-activator (Tat) acts through the transactivation response element RNA stem-loop to increase greatly the processivity of RNA polymerase II. Without Tat, transcription originating from the HIV promoter is attenuated. In this study, we demonstrate that transcriptional activation by Tat in vivo and in vitro requires the C-terminal domain (CTD) of RNA polymerase II. In contrast, the CTD is not required for basal transcription and for the formation of short, attenuated transcripts. Thus, trans-activation by Tat resembles enhancer- dependent activation of transcription. These results suggest that effects of Tat on the processivity of RNA polymerase II require proteins that are associated with the CTD and may result in the phosphorylation of the CTD.
AB - Human immunodeficiency virus (HIV)encoded trans-activator (Tat) acts through the transactivation response element RNA stem-loop to increase greatly the processivity of RNA polymerase II. Without Tat, transcription originating from the HIV promoter is attenuated. In this study, we demonstrate that transcriptional activation by Tat in vivo and in vitro requires the C-terminal domain (CTD) of RNA polymerase II. In contrast, the CTD is not required for basal transcription and for the formation of short, attenuated transcripts. Thus, trans-activation by Tat resembles enhancer- dependent activation of transcription. These results suggest that effects of Tat on the processivity of RNA polymerase II require proteins that are associated with the CTD and may result in the phosphorylation of the CTD.
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U2 - 10.1073/pnas.93.21.11575
DO - 10.1073/pnas.93.21.11575
M3 - Article
C2 - 8876177
AN - SCOPUS:0029969105
SN - 0027-8424
VL - 93
SP - 11575
EP - 11579
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -