Trafficking of MHC molecules to the cell surface creates dynamic protein patches

Daniel Blumenthal, Michael A Edidin, Levi A. Gheber

Research output: Contribution to journalArticle

Abstract

Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniformly distributed, but occur in patches in the plasma membrane. Here, we describe the temporal details of MHC-I patch formation and combine them with the spatial details, which we have described earlier, to yield a comprehensive quantitative description of patch formation. MHC-I is delivered to the plasma membrane in clathrin-coated vesicles, arriving at a rate of ~2.5×10-3 μm-1 min-1 (or about two arrivals per minute over the whole cell). The vesicles dock and fuse at non-random, apparently targeted, locations on the membrane and the newly delivered MHC-I molecules form patches that are a few hundred nanometers in diameter. The patches are maintained at steady state by a dynamic equilibrium between the rate of delivery and the rate of hindered diffusion of MHC-I molecules out of the patches (caused by components of the actin cytoskeleton).

Original languageEnglish (US)
Pages (from-to)3342-3350
Number of pages9
JournalJournal of Cell Science
Volume129
Issue number17
DOIs
StatePublished - 2016

Fingerprint

Major Histocompatibility Complex
Proteins
Cell Membrane
Clathrin-Coated Vesicles
Actin Cytoskeleton
Membrane Proteins
T-Lymphocytes
Membranes
Infection

Keywords

  • Fluorescence recovery after photobleaching
  • FRAP
  • Major histocompatibility complex class I
  • Membrane trafficking
  • MHC-I
  • Obstructed diffusion
  • TIRFM
  • Total internal reflection fluorescence microscopy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Trafficking of MHC molecules to the cell surface creates dynamic protein patches. / Blumenthal, Daniel; Edidin, Michael A; Gheber, Levi A.

In: Journal of Cell Science, Vol. 129, No. 17, 2016, p. 3342-3350.

Research output: Contribution to journalArticle

Blumenthal, Daniel ; Edidin, Michael A ; Gheber, Levi A. / Trafficking of MHC molecules to the cell surface creates dynamic protein patches. In: Journal of Cell Science. 2016 ; Vol. 129, No. 17. pp. 3342-3350.
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