Tracking the polymerization of DNA sensors and inflammasomes using FRET

Zachary Mazanek, Jungsan Sohn

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Upon activation, DNA sensors and inflammasomes often polymerize into filamentous oligomers, and transduce the incoming pathogenic signal via inducing the assembly of downstream filaments. Given the complexity of these supramolecular structures, kinetics and thermodynamics that govern their assembly mechanisms remain poorly understood. Here, we present a simple yet robust assay that can track the assembly of these filaments in a quantitative manner. This FRET-based measurement is cost effective and also amenable to high-throughput screening.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsJungsan Sohn
PublisherAcademic Press Inc.
Pages87-94
Number of pages8
ISBN (Print)9780128183595
DOIs
StatePublished - 2019

Publication series

NameMethods in Enzymology
Volume625
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • FRET
  • Inflammasomes
  • Kinetics
  • Polymerization
  • Quantitative assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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