@inbook{62d868e7c34044f282787cd6a3b47e32,
title = "Tracking the polymerization of DNA sensors and inflammasomes using FRET",
abstract = "Upon activation, DNA sensors and inflammasomes often polymerize into filamentous oligomers, and transduce the incoming pathogenic signal via inducing the assembly of downstream filaments. Given the complexity of these supramolecular structures, kinetics and thermodynamics that govern their assembly mechanisms remain poorly understood. Here, we present a simple yet robust assay that can track the assembly of these filaments in a quantitative manner. This FRET-based measurement is cost effective and also amenable to high-throughput screening.",
keywords = "FRET, Inflammasomes, Kinetics, Polymerization, Quantitative assay",
author = "Zachary Mazanek and Jungsan Sohn",
note = "Funding Information: This work was supported by NSF CAREER Award (1845003) to J.S. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mie.2019.06.006",
language = "English (US)",
isbn = "9780128183595",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "87--94",
editor = "Jungsan Sohn",
booktitle = "DNA Sensors and Inflammasomes",
}