Tracking the polymerization of DNA sensors and inflammasomes using FRET

Zachary Mazanek, Jungsan Sohn

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Upon activation, DNA sensors and inflammasomes often polymerize into filamentous oligomers, and transduce the incoming pathogenic signal via inducing the assembly of downstream filaments. Given the complexity of these supramolecular structures, kinetics and thermodynamics that govern their assembly mechanisms remain poorly understood. Here, we present a simple yet robust assay that can track the assembly of these filaments in a quantitative manner. This FRET-based measurement is cost effective and also amenable to high-throughput screening.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Inflammasomes
Thermodynamics
Polymerization
Costs and Cost Analysis
DNA
Sensors
Oligomers
Assays
Screening
Chemical activation
Throughput
Kinetics
Costs

Keywords

  • FRET
  • Inflammasomes
  • Kinetics
  • Polymerization
  • Quantitative assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Mazanek, Z., & Sohn, J. (2019). Tracking the polymerization of DNA sensors and inflammasomes using FRET. In Methods in Enzymology (Methods in Enzymology). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.06.006

Tracking the polymerization of DNA sensors and inflammasomes using FRET. / Mazanek, Zachary; Sohn, Jungsan.

Methods in Enzymology. Academic Press Inc., 2019. (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mazanek, Z & Sohn, J 2019, Tracking the polymerization of DNA sensors and inflammasomes using FRET. in Methods in Enzymology. Methods in Enzymology, Academic Press Inc. https://doi.org/10.1016/bs.mie.2019.06.006
Mazanek Z, Sohn J. Tracking the polymerization of DNA sensors and inflammasomes using FRET. In Methods in Enzymology. Academic Press Inc. 2019. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2019.06.006
Mazanek, Zachary ; Sohn, Jungsan. / Tracking the polymerization of DNA sensors and inflammasomes using FRET. Methods in Enzymology. Academic Press Inc., 2019. (Methods in Enzymology).
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