Tracking the intracellular path of fluorescently labeled DNA delivered by PEI nanocomplexes in live cells

Y. An, J. Suh, J. Hanes

Research output: Contribution to conferencePaper

Abstract

The tracking of the intracellular transport of polyethylenimine (PEI)/DNA nanocomplexes by fluorescently labeling the the PEI component of the gene was investigated. DNA was fluorescently labeled in the complexes with an intercalating dye, POPO-3, to determine if the DNa component of the PEI/DNA complex also follow the same intracellular route. At 24 h post-transfection, the signal from fluorescently labeled DNA was rarely detectable in positively transfected cells, and therefore, few PEI/DNA nanocomplexes were visible. The results show that DNA is dissociated fro PEI in positively transfected cells, resulting in faint diffuse fluorescence signals indistinguishable from cellular auto-fluorescence.

Original languageEnglish (US)
Pages1095-1096
Number of pages2
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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    An, Y., Suh, J., & Hanes, J. (2004). Tracking the intracellular path of fluorescently labeled DNA delivered by PEI nanocomplexes in live cells. 1095-1096. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.