Abstract
The tracking of the intracellular transport of polyethylenimine (PEI)/DNA nanocomplexes by fluorescently labeling the the PEI component of the gene was investigated. DNA was fluorescently labeled in the complexes with an intercalating dye, POPO-3, to determine if the DNa component of the PEI/DNA complex also follow the same intracellular route. At 24 h post-transfection, the signal from fluorescently labeled DNA was rarely detectable in positively transfected cells, and therefore, few PEI/DNA nanocomplexes were visible. The results show that DNA is dissociated fro PEI in positively transfected cells, resulting in faint diffuse fluorescence signals indistinguishable from cellular auto-fluorescence.
Original language | English (US) |
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Pages | 1095-1096 |
Number of pages | 2 |
State | Published - 2004 |
Event | 2004 AIChE Annual Meeting - Austin, TX, United States Duration: Nov 7 2004 → Nov 12 2004 |
Other
Other | 2004 AIChE Annual Meeting |
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Country/Territory | United States |
City | Austin, TX |
Period | 11/7/04 → 11/12/04 |
ASJC Scopus subject areas
- General Engineering