Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The innate immune system provides host defense against a variety of threats, including microbial infection and cellular damage. Pattern recognition receptors identify both pathogen and damage associated molecular motifs associated with these events. Nucleic acid is one such danger signal that is detected by a set of cytoplasmic sensors. Oligomerization is a key mode of activation shared by many of these sensors, who form large multimeric structures when activated. Microscopy is one of several techniques that facilitates study of these molecules, permitting visualization of oligomeric forms, and tracking of relevant signaling partners. In this chapter, we will discuss methods of generating and imaging cytoplasmic DNA sensors and inflammasome complexes by fluorescence microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsJungsan Sohn
PublisherAcademic Press Inc.
Pages253-267
Number of pages15
ISBN (Print)9780128183595
DOIs
StatePublished - 2019

Publication series

NameMethods in Enzymology
Volume625
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell imaging
  • DNA sensor
  • Immunofluorescence microscopy
  • Inflammasome
  • Innate immunity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Antiochos, B. (2019). Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy. In J. Sohn (Ed.), Methods in Enzymology (pp. 253-267). (Methods in Enzymology; Vol. 625). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.05.005