Tracking an embryonic L1 retrotransposition event

Eline T. Luning Prak, Allen W. Dodson, Evan A. Farkash, Haig H. Kazazian

Research output: Contribution to journalArticle

Abstract

Long interspersed nuclear elements 1 (L1) are active retrotransposons that reside in many species, including humans and rodents. L1 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic location. We have tagged an active human L1 element (L1RP) with a gene encoding enhanced GFP (EGFP). Expression of GFP occurs only if L1-EGFP has undergone a cycle of transcription, reverse transcription, and integration into a transcriptionally permissive genomic region. We show here that L1-EGFP can undergo retrotransposition in vivo and produce fluorescence in mouse testis. The retrotransposition event characterized here has occurred at a very early stage in the development of an L1-EGFP transgenic founder mouse.

Original languageEnglish (US)
Pages (from-to)1832-1837
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number4
DOIs
StatePublished - Feb 18 2003
Externally publishedYes

Keywords

  • Enhanced GFP
  • L1 transgenic mice

ASJC Scopus subject areas

  • General

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