Toxicity potential of absorbed-retained ethylene oxide residues in culture dishes on embryo development in vitro.

M. C. Schiewe, P. M. Schmidt, M. Bush, D. E. Wildt

Research output: Contribution to journalArticle

Abstract

Four hundred eight-cell mouse embryos were cultured in vitro in either polystyrene (plastic) dishes (Exp. 1) or watch glasses (Exp. 2) to analyze the toxicity potential of absorbed-retained ethylene oxide (EtO). Culture dishes were gas-sterilized with Anprolene equipment and allowed to aerate (21 C) for varying durations. Post-sterilization EtO residues, as determined by weight measurement, were eluted from polystyrene dishes at an exponential rate. After 1 wk of aeration, .625 mg EtO was retained/g of polystyrene product. To determine the effect of EtO residue on in vitro culture, embryos were collected from superovulated donor mice (C57BL/6N), pooled in a modified Dulbecco's phosphate buffered saline (PBI) medium and then randomly allotted to dishes subjected to various aeration durations. Embryos were cultured in Whitten's medium +3 mg/ml bovine serum albumin (BSA) under a humidified 5% CO2 in air atmosphere at 37 C. Gross assessments of embryo development were made using standard morphology and quality grading systems and a fluorescein diacetate staining assay. In Exp. 1, embryo development was retarded at the 8- to 16-cell stage when less than or equal to 12 h aeration of polystyrene dishes was permitted. Aeration for 24 and 36 h resulted in suboptimal embryo development with fewer (P less than .01) blastocysts and greater degeneration rates at 48 h of in vitro culture compared with the control (manufacturer-packaged dishes, no EtO) and 1-wk aeration treatment groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)1610-1618
Number of pages9
JournalJournal of Animal Science
Volume60
Issue number6
StatePublished - Jun 1985
Externally publishedYes

Fingerprint

ethylene oxide
Ethylene Oxide
aeration
Embryonic Development
Polystyrenes
polystyrenes
embryogenesis
toxicity
embryo (animal)
Embryonic Structures
in vitro culture
duration
mice
Blastocyst
fluorescein
bovine serum albumin
Bovine Serum Albumin
Atmosphere
Inbred C57BL Mouse
blastocyst

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Toxicity potential of absorbed-retained ethylene oxide residues in culture dishes on embryo development in vitro. / Schiewe, M. C.; Schmidt, P. M.; Bush, M.; Wildt, D. E.

In: Journal of Animal Science, Vol. 60, No. 6, 06.1985, p. 1610-1618.

Research output: Contribution to journalArticle

Schiewe, M. C. ; Schmidt, P. M. ; Bush, M. ; Wildt, D. E. / Toxicity potential of absorbed-retained ethylene oxide residues in culture dishes on embryo development in vitro. In: Journal of Animal Science. 1985 ; Vol. 60, No. 6. pp. 1610-1618.
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