TY - JOUR
T1 - Toxic waste disposal in Escherichia coli
AU - Helling, Robert B.
AU - Janes, Brian K.
AU - Kimball, Heather
AU - Tran, Timothy
AU - Bundesmann, Michael
AU - Check, Pietra
AU - Phelan, Darcy
AU - Miller, Charles
PY - 2002
Y1 - 2002
N2 - About 10% of the nalidixic acid-resistant (Nalr) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nalr mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.
AB - About 10% of the nalidixic acid-resistant (Nalr) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nalr mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.
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U2 - 10.1128/JB.184.13.3699-3703.2002
DO - 10.1128/JB.184.13.3699-3703.2002
M3 - Article
C2 - 12057966
AN - SCOPUS:0036285420
SN - 0021-9193
VL - 184
SP - 3699
EP - 3703
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 13
ER -