Toxic waste disposal in Escherichia coli

Robert B. Helling, Brian K. Janes, Heather Kimball, Timothy Tran, Michael Bundesmann, Pietra Check, Darcy Phelan, Charles Miller

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


About 10% of the nalidixic acid-resistant (Nalr) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nalr mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.

Original languageEnglish (US)
Pages (from-to)3699-3703
Number of pages5
JournalJournal of bacteriology
Issue number13
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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