Toxic effects of high dose cyanate administration in rodents

B. P. Alter, Y. W. Kan, D. G. Nathan

Research output: Contribution to journalArticle

Abstract

Cyanate inhibits hemoglobin synthesis in vitro. In vivo studies were performed to determine the effect of the drug on erythropoiesis. Adult female rats received daily intraperitoneal injections of 400 μmoles of cyanate, leading to over 3 CNO/Hb by 3 wk. Controls received sodium chloride. DPG was measured in a small number of animals and declined from 5.5 to 4.1 mmoles/l (p <0.02). At 4 wk, Hb, Hct, and red cell counts in cardiac blood of the treated group were all higher (p <0.05); MCHC was normal. Mean plasma iron was 175 in treated versus 129 μg/100 ml in controls. Plasma iron turnover in mg/day increased from 0.143 to 0.260 (p <0.01), as did erythron iron turnover, from 0.096 to 0.195 mg/day (p <0.05). Thus the erythroid marrow of cyanate treated animals could respond to carbamylation induced increase in oxygen affinity, though the adequacy of the response with respect to the increase in oxygen affinity is difficult to assess. Furthermore, severe clinical toxicity was observed. Treated animals lost a mean of 62 g compared with a gain of 25 g in controls. 25% died by 4 wk. The neuromuscular system appeared to be more sensitive to high dose in vivo cyanate administration than the erythropoietic system.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalBlood
Volume43
Issue number1
StatePublished - 1974
Externally publishedYes

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Cyanates
Poisons
Rodentia
Animals
Iron
Oxygen
Plasmas
Erythropoiesis
Blood Group Antigens
Intraperitoneal Injections
Sodium Chloride
Toxicity
Rats
Hemoglobins
Blood
Cell Count
Bone Marrow
Cells
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Hematology

Cite this

Alter, B. P., Kan, Y. W., & Nathan, D. G. (1974). Toxic effects of high dose cyanate administration in rodents. Blood, 43(1), 69-77.

Toxic effects of high dose cyanate administration in rodents. / Alter, B. P.; Kan, Y. W.; Nathan, D. G.

In: Blood, Vol. 43, No. 1, 1974, p. 69-77.

Research output: Contribution to journalArticle

Alter, BP, Kan, YW & Nathan, DG 1974, 'Toxic effects of high dose cyanate administration in rodents', Blood, vol. 43, no. 1, pp. 69-77.
Alter BP, Kan YW, Nathan DG. Toxic effects of high dose cyanate administration in rodents. Blood. 1974;43(1):69-77.
Alter, B. P. ; Kan, Y. W. ; Nathan, D. G. / Toxic effects of high dose cyanate administration in rodents. In: Blood. 1974 ; Vol. 43, No. 1. pp. 69-77.
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