@article{88ab5af7b9f340dcb6ad75c2756db6bb,
title = "Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration",
abstract = "Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV refractive index, epitope abundance, size and concentration. Due to their predominant use among EV researchers, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.",
keywords = "Calibration, exosomes, extracellular vesicles, microvesicles, optical analysis, quality control, reference materials, standardization, validation",
author = "Welsh, {Joshua A.} and {van der Pol}, Edwin and Bettin, {Britta A.} and Carter, {David R.F.} and An Hendrix and Metka Lenassi and Langlois, {Marc Andr{\'e}} and Alicia Llorente and {van de Nes}, {Arthur S.} and Rienk Nieuwland and Vera Tang and Lili Wang and Witwer, {Kenneth W.} and Jones, {Jennifer C.}",
note = "Funding Information: JAW and JCJ are supported by the U.S. National Institutes of Health, National Cancer Institute, 1ZIA-BC011502, and the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute, Center for Cancer Research (JAW). JCJ acknowledges NIH ZIA BC011502, NIH ZIA BC011503, NIH U01 HL126497, NIH R01 CA218500, NIH UG3 TR002881, FNIH Ruth Anne Cafritz Award and Prostate Cancer Foundation YIA funding. KWW acknowledges NIH DA040385, DA047807, AI144997, MH118164 and UG3CA241694, as well as the Michael J. Fox Foundation. JAW is an ISAC Marylou Ingram Scholar 2019-2023. VAT is an ISAC Shared Resource Lab Emerging Leader 2018-2022. ML is supported by the Slovenian Research Agency (ARRS) projects P1-0170 and J3-9255. ALL is supported by The Norwegian Research Council and by the South-Eastern Norway Regional Health Authority. EvdP is supported by the Netherlands Organisation for Scientific Research—Domain Applied and Engineering Sciences (NWO-TTW), research program VENI 15924. BB is supported by European Metrology Research Programme Joint Research Project 18HLT01: METVES II. DRFC is supported by the BBSRC (BB/P006205/1) and Cancer Research UK (A28052);Biotechnology and Biological Sciences Research Council [BB/P006205/1];Cancer Research UK [A28052];European Metrology Programme for Innovation and Research [18HLT01];Michael J. Fox Foundation for Parkinson{\textquoteright}s Research [NA];National Cancer Institute [1ZIA-BC011502];National Cancer Institute [U01 HL126497];National Cancer Institute [R01 CA218500];National Cancer Institute [UG3 TR002881];National Department of Health [ZIA BC011503];National Department of Health [ZIA BC011502,];National Institutes of Health [DA047807];National Institutes of Health [UG3CA241694];National Institutes of Health [DA040385];National Institutes of Health [MH118164];National Institutes of Health [AI144997];Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NL) [VENI 15924];South-Eastern Norway Regional Health Authority [NA];Slovenian Research Agency [J3-9255];Slovenian Research Agency [P1-0170];Norwegian Research Council [NA];FNIH Ruth Anne Cafritz Award [NA];Prostate Cancer Foundation [NA]. The authors thank John P. Nolan and George Daaboul from Cellarcus Biosciences and NanoView, respectively, for providing reagent information and constructive feedback. Publisher Copyright: {\textcopyright} 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.",
year = "2020",
month = jan,
day = "1",
doi = "10.1080/20013078.2020.1816641",
language = "English (US)",
volume = "9",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Taylor and Francis Ltd.",
number = "1",
}