Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration

Joshua A. Welsh, Edwin van der Pol, Britta A. Bettin, David R.F. Carter, An Hendrix, Metka Lenassi, Marc André Langlois, Alicia Llorente, Arthur S. van de Nes, Rienk Nieuwland, Vera Tang, Lili Wang, Kenneth W. Witwer, Jennifer C. Jones

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations

Abstract

Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV refractive index, epitope abundance, size and concentration. Due to their predominant use among EV researchers, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.

Original languageEnglish (US)
Article number1816641
JournalJournal of Extracellular Vesicles
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2020

Keywords

  • Calibration
  • exosomes
  • extracellular vesicles
  • microvesicles
  • optical analysis
  • quality control
  • reference materials
  • standardization
  • validation

ASJC Scopus subject areas

  • Histology
  • Cell Biology

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