Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster

Characterization of male-sterile mutants generated by single P element mutagenesis

D. H. Castrillon, P. Gonczy, S. Alexander, R. Rawson, Charles G Eberhart, S. Viswanathan, S. DiNardo, S. A. Wasserman

Research output: Contribution to journalArticle

Abstract

We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well as complementation tests using available chromosomal deficiencies, indicate that the insertions are responsible for the mutant phenotypes. These mutations represent 63 complementation groups, 58 of which are required for spermatogenesis. Phenotypes of the spermatogenesis mutants were analyzed by light microscopy. Mutations in 12 loci affect germline proliferation, spermatocyte growth, or meiosis. Mutations in 46 other loci disrupt differentiation and maturation of spermatids into motile sperm. This collection of male-sterile mutants provides the basis for a molecular genetic analysis of spermatogenesis.

Original languageEnglish (US)
Pages (from-to)489-505
Number of pages17
JournalGenetics
Volume135
Issue number2
StatePublished - 1993
Externally publishedYes

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Spermatogenesis
Drosophila melanogaster
Mutagenesis
Molecular Biology
Mutation
Genetic Complementation Test
Phenotype
Spermatocytes
Spermatids
Meiosis
Spermatozoa
Microscopy
Light
Growth

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster : Characterization of male-sterile mutants generated by single P element mutagenesis. / Castrillon, D. H.; Gonczy, P.; Alexander, S.; Rawson, R.; Eberhart, Charles G; Viswanathan, S.; DiNardo, S.; Wasserman, S. A.

In: Genetics, Vol. 135, No. 2, 1993, p. 489-505.

Research output: Contribution to journalArticle

Castrillon, DH, Gonczy, P, Alexander, S, Rawson, R, Eberhart, CG, Viswanathan, S, DiNardo, S & Wasserman, SA 1993, 'Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: Characterization of male-sterile mutants generated by single P element mutagenesis', Genetics, vol. 135, no. 2, pp. 489-505.
Castrillon, D. H. ; Gonczy, P. ; Alexander, S. ; Rawson, R. ; Eberhart, Charles G ; Viswanathan, S. ; DiNardo, S. ; Wasserman, S. A. / Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster : Characterization of male-sterile mutants generated by single P element mutagenesis. In: Genetics. 1993 ; Vol. 135, No. 2. pp. 489-505.
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