Total cytokine immunoassay: A more accurate method of cytokine measurement?

Debra Malone, Lena M. Napolitano, Thomas Genuit, Grant V. Bochicchio, Kerry Kole, Thomas M. Scalea

Research output: Contribution to journalArticle

Abstract

Background: Cytokines signal the normal processes of inflammation and repair in all organs, yet the aberrant expression of these peptide mediators is associated with significant organ dysfunction. The accurate measurement of cytokines is therefore critical. In this study, we sought to investigate the alterations in cytokine expression early after trauma in humans using a new competitive binding immunoassay that measures both free and bound cytokine and compare this with standard enzyme-linked immunosorbent assay (ELISA), which measures only free cytokine. Methods: Peripheral blood was obtained from trauma patients at admission. Exclusion criteria were transfers, death within 24 hours, pregnancy, known acquired immunodeficiency syndrome, chemotherapy, transplant, or other chronic immune disorder. "Total" cytokine immunoassay was compared with ELISA for cytokines (interleukin [IL]-1, IL-6, and IL-10) measured in serum. Results: Cytokine concentrations measured by total immunoassay were significantly higher (10- to 500-fold increase) than those measured by ELISA, and correlation between the two methods was poor (r2 = 0.193 for IL-10). No significant differences in mean serum cytokine concentrations were noted between trauma patients and normal controls for IL-1 (56 vs. 37 pg/mL), IL-6 (16 vs. 25 pg/mL), and IL-10 (4 vs. 26 pg/mL) using the ELISA method. In contrast, trauma patients had significantly higher serum concentrations of IL-1 (3,320 vs. 1,470 pg/mL, p <0.05), IL-6 (2,415 vs. 1,048 pg/mL, p <0.05), and IL-10 (2,307 vs. 1,480 pg/mL, p <0.05) at admission compared with normal controls using total cytokine immunoassays. Conclusion: Cytokine measurements in peripheral blood in trauma patients and normal controls are significantly (10- to 500-fold) higher when using a total cytokine assay that measures both free and bound cytokine. Competitive immunoassays may be the method of choice when measuring endogenous cytokine levels in biologic fluids, and new normal ranges for cytokines must be established for future accurate research in critical care and trauma.

Original languageEnglish (US)
Pages (from-to)821-825
Number of pages5
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume50
Issue number5
StatePublished - 2001
Externally publishedYes

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Immunoassay
Cytokines
Interleukin-10
Wounds and Injuries
Enzyme-Linked Immunosorbent Assay
Interleukin-1
Interleukin-6
Interleukin-2
Interleukin-16
Serum
Competitive Binding
Interleukin-3
Patient Admission
Immune System Diseases
Critical Care
Interleukin-4
Reference Values
Acquired Immunodeficiency Syndrome

Keywords

  • Cytokines
  • Immunoassay
  • Inflammation
  • Injury
  • Multiple organ failure
  • Trauma

ASJC Scopus subject areas

  • Surgery

Cite this

Malone, D., Napolitano, L. M., Genuit, T., Bochicchio, G. V., Kole, K., & Scalea, T. M. (2001). Total cytokine immunoassay: A more accurate method of cytokine measurement? Journal of Trauma - Injury, Infection and Critical Care, 50(5), 821-825.

Total cytokine immunoassay : A more accurate method of cytokine measurement? / Malone, Debra; Napolitano, Lena M.; Genuit, Thomas; Bochicchio, Grant V.; Kole, Kerry; Scalea, Thomas M.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 50, No. 5, 2001, p. 821-825.

Research output: Contribution to journalArticle

Malone, D, Napolitano, LM, Genuit, T, Bochicchio, GV, Kole, K & Scalea, TM 2001, 'Total cytokine immunoassay: A more accurate method of cytokine measurement?', Journal of Trauma - Injury, Infection and Critical Care, vol. 50, no. 5, pp. 821-825.
Malone D, Napolitano LM, Genuit T, Bochicchio GV, Kole K, Scalea TM. Total cytokine immunoassay: A more accurate method of cytokine measurement? Journal of Trauma - Injury, Infection and Critical Care. 2001;50(5):821-825.
Malone, Debra ; Napolitano, Lena M. ; Genuit, Thomas ; Bochicchio, Grant V. ; Kole, Kerry ; Scalea, Thomas M. / Total cytokine immunoassay : A more accurate method of cytokine measurement?. In: Journal of Trauma - Injury, Infection and Critical Care. 2001 ; Vol. 50, No. 5. pp. 821-825.
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