TY - JOUR
T1 - Topical tissue factor pathway inhibitor improves free-flap survival in a model simulating free-flap errors
AU - Ozbeck, M. R.
AU - Brown, D. M.
AU - Deune, E. G.
AU - Lantieri, L. A.
AU - Kania, N. M.
AU - Pasia, E. N.
AU - Cooley, B. C.
AU - Wun, T. C.
AU - Khouri, R. K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - Free flap failure is frequently due to tension, twisting, kinking, or compression of the vascular pedicle after the anastomosis is completed A rabbit model simulating these errors was used to evaluate the capacity of topically-applied tissue factor pathway inhibitor (TFPI) to prevent microvascular thrombosis. The rabbit ear was isolated on the central artery and vein the artery was transected, shortened, repaired, and twisted 360° around the vein immediately following the anastomosis, TFPI in concentrations of 1, 4, 10, or 40 μg/ml was irrigated across the lumen. Topically-applied control buffer and heparin (50 U/ml) were compared to TFPI. Treatment with control buffer resulted in a 20 percent survival rate topically-applied heparin improved the survival rate to 60 percent (p <0.05) in contrast. TFPI in concentrations of 4, 10, and 40 μg/ml yielded survival rates of 89, 100, and 97 percent, respectively. This was significantly greater than the heparin-treated ears (p <0.05). TFPI in a concentration of 40 μg/ml was effective in preventing arterial thrombosis when applied for as little as 30 sec; 4 μg/ml was effective in preventing thrombosis when applied for 10 min. These results support the use of TFPI as a topical irrigation solution to help prevent microvascular arterial thrombosis in free-flap surgery.
AB - Free flap failure is frequently due to tension, twisting, kinking, or compression of the vascular pedicle after the anastomosis is completed A rabbit model simulating these errors was used to evaluate the capacity of topically-applied tissue factor pathway inhibitor (TFPI) to prevent microvascular thrombosis. The rabbit ear was isolated on the central artery and vein the artery was transected, shortened, repaired, and twisted 360° around the vein immediately following the anastomosis, TFPI in concentrations of 1, 4, 10, or 40 μg/ml was irrigated across the lumen. Topically-applied control buffer and heparin (50 U/ml) were compared to TFPI. Treatment with control buffer resulted in a 20 percent survival rate topically-applied heparin improved the survival rate to 60 percent (p <0.05) in contrast. TFPI in concentrations of 4, 10, and 40 μg/ml yielded survival rates of 89, 100, and 97 percent, respectively. This was significantly greater than the heparin-treated ears (p <0.05). TFPI in a concentration of 40 μg/ml was effective in preventing arterial thrombosis when applied for as little as 30 sec; 4 μg/ml was effective in preventing thrombosis when applied for 10 min. These results support the use of TFPI as a topical irrigation solution to help prevent microvascular arterial thrombosis in free-flap surgery.
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U2 - 10.1055/s-2007-1006529
DO - 10.1055/s-2007-1006529
M3 - Article
C2 - 7650643
AN - SCOPUS:0029065430
SN - 0743-684X
VL - 11
SP - 185
EP - 188
JO - Journal of reconstructive microsurgery
JF - Journal of reconstructive microsurgery
IS - 3
ER -