Abstract
PURPOSE. Allergic conjunctivitis (AC) is an immunoglobulin E (IgE)-mediated and helper T cell 2 (Th2)–cell–mediated disease characterized by conjunctival eosinophilic infiltration. Previous study shows that IL-28A had anti-allergic activity in airway disease. In this study, we examined the effect of IL-28A on a mouse model of ovalbumin (OVA)-induced experimental allergic conjunctivitis (EAC). METHODS. Mouse EAC was induced by topical application of OVA after intraperitoneal (IP) sensitization with OVA in aluminum hydroxide (ALUM). Interleukin-28A was administered 1 hour before OVA challenge. Allergic conjunctivitis symptoms, eosinophil infiltration in the conjunctiva, antigen-specific IgE in the serum, and Th2 cytokine production by lymph node cells and splenocytes were subsequently analyzed. RESULTS. Topical application of IL-28A to OVA-induced EAC reduced clinical symptoms, serum OVA-specific IgE, and the infiltration of eosinophils in the conjunctiva. In addition, topical administration of IL-28A suppressed the expression of IL-4, IL-5, and IL-13 (Th2-type cytokine) but promoted the expression of IFN-γ (Th1-type cytokine) by splenocytes and cervical lymph node cells in EAC mice. Immunofluorescence staining showed decrease expression of IL-4 and IL-5 in IL-28A–treated EAC conjunctiva. CONCLUSIONS. Interleukin-28A shows therapeutic potential for allergic conjunctival inflammation.
Original language | English (US) |
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Pages (from-to) | 604-610 |
Number of pages | 7 |
Journal | Investigative Ophthalmology and Visual Science |
Volume | 57 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2016 |
Externally published | Yes |
Keywords
- Allergic conjunctivitis
- Eosinophilic
- Eotaxin
- Immunoglobulin E
- Interleukin-28A
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience