Topical application of interleukin-28A attenuates allergic conjunctivitis in an ovalbumin-induced mouse model

Jianping Chen, Jing Zhang, Ruijuan Zhao, Jiayi Jin, Ying Yu, Weihua Li, Wencong Wang, Hongyan Zhou, Shao Bo Su

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE. Allergic conjunctivitis (AC) is an immunoglobulin E (IgE)-mediated and helper T cell 2 (Th2)–cell–mediated disease characterized by conjunctival eosinophilic infiltration. Previous study shows that IL-28A had anti-allergic activity in airway disease. In this study, we examined the effect of IL-28A on a mouse model of ovalbumin (OVA)-induced experimental allergic conjunctivitis (EAC). METHODS. Mouse EAC was induced by topical application of OVA after intraperitoneal (IP) sensitization with OVA in aluminum hydroxide (ALUM). Interleukin-28A was administered 1 hour before OVA challenge. Allergic conjunctivitis symptoms, eosinophil infiltration in the conjunctiva, antigen-specific IgE in the serum, and Th2 cytokine production by lymph node cells and splenocytes were subsequently analyzed. RESULTS. Topical application of IL-28A to OVA-induced EAC reduced clinical symptoms, serum OVA-specific IgE, and the infiltration of eosinophils in the conjunctiva. In addition, topical administration of IL-28A suppressed the expression of IL-4, IL-5, and IL-13 (Th2-type cytokine) but promoted the expression of IFN-γ (Th1-type cytokine) by splenocytes and cervical lymph node cells in EAC mice. Immunofluorescence staining showed decrease expression of IL-4 and IL-5 in IL-28A–treated EAC conjunctiva. CONCLUSIONS. Interleukin-28A shows therapeutic potential for allergic conjunctival inflammation.

Original languageEnglish (US)
Pages (from-to)604-610
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Keywords

  • Allergic conjunctivitis
  • Eosinophilic
  • Eotaxin
  • Immunoglobulin E
  • Interleukin-28A

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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